※ Data Summary

 Type  Protein  Site
 Acetylation  20,088  58,563
 Butyrylation  9  39
 Crotonylation  11  58
 Glycation  72  323
 Malonylation  24  35
 Methylation  745  1,144
 Phosphoglycerylation  110  141
 Propionylation  9  53
 Pupylation  182  215
 Succinylation  897  2,523
 Sumoylation  538  928
 Ubiquitination  32,429  139,950
 Total  45,748  189,919
 Species  Protein  Site
 Homo sapiens  21,782  108,009
 Mus musculus  10,187  38,855
 Rattus norvegicus  5,152  16,096
 Drosophila melanogaster  1,224  2,409
 Cavia porcellus  263  991
 Plasmodium falciparum  230  426
 Toxoplasma gondii  470  715
 Saccharomyces cerevisiae  2,617  9,606
 Arabidopsis thaliana  213  315
 Escherichia coli  1,834  8,734
 Mycoplasma pneumoniae  220  717
 Rhodopseudomonas palustris  243  290
 Others  1,313  2,756
 Total  45,748  189,919


In CPLM database, we tried to make it powerful and convenient to be used. The online service and local packages were provided. This USAGE is prepared for the online service. For usage of local packages, please download the Manual. The CPLM 2.0 provide the search option and advance options.

1. Substrate Search. You can input one or multiple keywords (separated by space character) to search the CPLM database. The search fields including CPLM ID, UniProt Accession, Protein Name, Protein Alias, Gene Name, Gene Alias, Species, and Function.

EXAMPLE: Please click on the "Example" button to search "p53" in Gene Name field. By clicking on the "Submit" button, the related proteins will be shown.

2. Browse. Two simple ways have been provided for users to browse lysine modified protein in the database. Users can browse by species or by modification type. Type browse allows user to select interested type. Species browse allows user to choose interested species.

3. Advanced Options. Three advance options are provided, including advance search, browse, BLAST search.

(1) Advanced search allows you to input up to two terms to find the information more specifically. The querying fields can be empty if less terms are needed. The two terms could be connected by the following operators:

exclude: If selected, the term following this operator must be not contained in the specified field(s)
and: the term following this operator has to be included in the specified field(s)
or: either the preceding or the following term to this operator should occur in the specified field(s)

EXAMPLE: You can click on the "Example" button to load an instance, which could search p53 in human. The human p53 (CPLM-000598) will be shown by clicking on the "Submit" button.

(2) Multiple search could be used to find a number of proteins such as a protein list. You can input keyword list, for example, a list of UniProt IDs to search the database. The list should be inputed as one keyword one line.

EXAMPLE: You can click on the "Example" button to load two protein ID including P68363 and P42229. By clicking on the "Submit" button, you can find Signal transducer and activator of transcription 5A and Tubulin alpha-1B chain.

(3) BLAST search could be used to find the specific protein and/or related homologues by sequence alignment. This search-option will help you to find the querying protein accurately and fast. Only one protein sequence in FASTA format is allowed per time. The E-value threshold could be user-defined, while the default is 0.01.

EXAMPLE: You can click on the "Example" button to load the protein sequence of human Histone H4. By clicking on the "Submit" button, you can find the related homologues in other species.