CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007198
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitochondrial respiratory chain complexes assembly protein AFG3 
Protein Synonyms/Alias
 ATPase family gene 3 protein; Tat-binding homolog 10 
Gene Name
 AFG3 
Gene Synonyms/Alias
 YTA10; YER017C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
706LLTKNLDKVDLVAKEacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Acts as a component of the m-AAA protease complex which is a ATP-dependent metalloprotease mediating degradation of non- assembled mitochondrial inner membrane proteins. The complex is necessary for the assembly of mitochondrial respiratory chain and ATPase complexes. Function both in post-translational assembly and in the turnover of mistranslated or misfolded polypeptides. 
Sequence Annotation
 NP_BIND 328 335 ATP (Potential).
 ACT_SITE 559 559 By similarity.
 METAL 558 558 Zinc; catalytic (By similarity).
 METAL 562 562 Zinc; catalytic (By similarity).
 METAL 634 634 Zinc; catalytic (By similarity).  
Keyword
 ATP-binding; Complete proteome; Hydrolase; Membrane; Metal-binding; Metalloprotease; Mitochondrion; Nucleotide-binding; Protease; Reference proteome; Transmembrane; Transmembrane helix; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 761 AA 
Protein Sequence
MMMWQRYARG APRSLTSLSF GKASRISTVK PVLRSRMPVH QRLQTLSGLA TRNTIHRSTQ 60
IRSFHISWTR LNENRPNKEG EGKNNGNKDN NSNKEDGKDK RNEFGSLSEY FRSKEFANTM 120
FLTIGFTIIF TLLTPSSNNS GDDSNRVLTF QDFKTKYLEK GLVSKIYVVN KFLVEAELVN 180
TKQVVSFTIG SVDIFEEQMD QIQDLLNIPP RDRIPIKYIE RSSPFTFLFP FLPTIILLGG 240
LYFITRKINS SPPNANGGGG GGLGGMFNVG KSRAKLFNKE TDIKISFKNV AGCDEAKQEI 300
MEFVHFLKNP GKYTKLGAKI PRGAILSGPP GTGKTLLAKA TAGEANVPFL SVSGSEFVEM 360
FVGVGASRVR DLFTQARSMA PSIIFIDEID AIGKERGKGG ALGGANDERE ATLNQLLVEM 420
DGFTTSDQVV VLAGTNRPDV LDNALMRPGR FDRHIQIDSP DVNGRQQIYL VHLKRLNLDP 480
LLTDDMNNLS GKLATLTPGF TGADIANACN EAALIAARHN DPYITIHHFE QAIERVIAGL 540
EKKTRVLSKE EKRSVAYHEA GHAVCGWFLK YADPLLKVSI IPRGQGALGY AQYLPPDQYL 600
ISEEQFRHRM IMALGGRVSE ELHFPSVTSG AHDDFKKVTQ MANAMVTSLG MSPKIGYLSF 660
DQNDGNFKVN KPFSNKTART IDLEVKSIVD DAHRACTELL TKNLDKVDLV AKELLRKEAI 720
TREDMIRLLG PRPFKERNEA FEKYLDPKSN TEPPEAPAAT N 761 
Gene Ontology
 GO:0005745; C:m-AAA complex; IDA:SGD.
 GO:0097002; C:mitochondrial inner boundary membrane; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; IDA:SGD.
 GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
 GO:0008237; F:metallopeptidase activity; IMP:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0002181; P:cytoplasmic translation; IMP:SGD.
 GO:0030163; P:protein catabolic process; IEA:InterPro.
 GO:0006461; P:protein complex assembly; IMP:SGD.
 GO:0045041; P:protein import into mitochondrial intermembrane space; TAS:SGD.
 GO:0006508; P:proteolysis; IMP:SGD.
 GO:0001302; P:replicative cell aging; IMP:SGD.
 GO:0006465; P:signal peptide processing; IMP:SGD. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR005936; FtsH.
 IPR027417; P-loop_NTPase.
 IPR011546; Pept_M41_FtsH_extracell.
 IPR000642; Peptidase_M41. 
Pfam
 PF00004; AAA
 PF06480; FtsH_ext
 PF01434; Peptidase_M41 
SMART
 SM00382; AAA 
PROSITE
 PS00674; AAA 
PRINTS