CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012327
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hypermethylated in cancer 1 protein 
Protein Synonyms/Alias
 Hic-1; Zinc finger and BTB domain-containing protein 29 
Gene Name
 HIC1 
Gene Synonyms/Alias
 ZBTB29 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
314SLLYRWMKHEPGLGSsumoylation[1]
Reference
 [1] An acetylation/deacetylation-SUMOylation switch through a phylogenetically conserved psiKXEP motif in the tumor suppressor HIC1 regulates transcriptional repression activity.
 Stankovic-Valentin N, Deltour S, Seeler J, Pinte S, Vergoten G, Guérardel C, Dejean A, Leprince D.
 Mol Cell Biol. 2007 Apr;27(7):2661-75. [PMID: 17283066] 
Functional Description
 Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1. The regulation of SIRT1 transcription in response to nutrient deprivation seems to involve CTBP1. In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells. Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF- responsive genes. Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex. Probably represses transcription from CXCR7, FGFBP1 and EFNA1. 
Sequence Annotation
 DOMAIN 47 110 BTB.
 ZN_FING 439 459 C2H2-type 1.
 ZN_FING 509 529 C2H2-type 2.
 ZN_FING 537 557 C2H2-type 3.
 ZN_FING 565 585 C2H2-type 4.
 ZN_FING 593 613 C2H2-type 5.
 REGION 154 315 Mediates HDAC-dependent transcriptional
 REGION 241 247 Interaction with CTBP1.
 MOD_RES 313 313 Phosphoserine (By similarity).
 MOD_RES 333 333 N6-acetyllysine; alternate.
 CROSSLNK 333 333 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Developmental protein; DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Tumor suppressor; Ubl conjugation; Wnt signaling pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 733 AA 
Protein Sequence
MLDTMEAPGH SRQLLLQLNN QRTKGFLCDV IIVVQNALFR AHKNVLAASS AYLKSLVVHD 60
NLLNLDHDMV SPAVFRLVLD FIYTGRLADG AEAAAAAAVA PGAEPSLGAV LAAASYLQIP 120
DLVALCKKRL KRHGKYCHLR GGGGGGGGYA PYGRPGRGLR AATPVIQACY PSPVGPPPPP 180
AAEPPSGPEA AVNTHCAELY ASGPGPAAAL CASERRCSPL CGLDLSKKSP PGSAAPERPL 240
AERELPPRPD SPPSAGPAAY KEPPLALPSL PPLPFQKLEE AAPPSDPFRG GSGSPGPEPP 300
GRPDGPSLLY RWMKHEPGLG SYGDELGRER GSPSERCEER GGDAAVSPGG PPLGLAPPPR 360
YPGSLDGPGA GGDGDDYKSS SEETGSSEDP SPPGGHLEGY PCPHLAYGEP ESFGDNLYVC 420
IPCGKGFPSS EQLNAHVEAH VEEEEALYGR AEAAEVAAGA AGLGPPFGGG GDKVAGAPGG 480
LGELLRPYRC ASCDKSYKDP ATLRQHEKTH WLTRPYPCTI CGKKFTQRGT MTRHMRSHLG 540
LKPFACDACG MRFTRQYRLT EHMRIHSGEK PYECQVCGGK FAQQRNLISH MKMHAVGGAA 600
GAAGALAGLG GLPGVPGPDG KGKLDFPEGV FAVARLTAEQ LSLKQQDKAA AAELLAQTTH 660
FLHDPKVALE SLYPLAKFTA ELGLSPDKAA EVLSQGAHLA AGPDGRTIDR FSPT 714 
Gene Ontology
 GO:0000785; C:chromatin; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0042826; F:histone deacetylase binding; IDA:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0008630; P:intrinsic apoptotic signaling pathway in response to DNA damage; IDA:UniProtKB.
 GO:0007275; P:multicellular organismal development; NAS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0030178; P:negative regulation of Wnt receptor signaling pathway; IDA:UniProtKB.
 GO:0043517; P:positive regulation of DNA damage response, signal transduction by p53 class mediator; ISS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 
Interpro
 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF00651; BTB
 PF00096; zf-C2H2 
SMART
 SM00225; BTB
 SM00355; ZnF_C2H2 
PROSITE
 PS50097; BTB
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS