CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008889
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ephrin type-B receptor 3 
Protein Synonyms/Alias
 Developmental kinase 5; mDK-5; Tyrosine-protein kinase receptor SEK-4 
Gene Name
 Ephb3 
Gene Synonyms/Alias
 Etk2; Mdk5; Sek4 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
889QIVNTLDKLIRNAASubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. Beside its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt. 
Sequence Annotation
 DOMAIN 31 209 Eph LBD.
 DOMAIN 332 438 Fibronectin type-III 1.
 DOMAIN 448 539 Fibronectin type-III 2.
 DOMAIN 628 891 Protein kinase.
 DOMAIN 920 984 SAM.
 NP_BIND 634 642 ATP (By similarity).
 MOTIF 991 993 PDZ-binding (Potential).
 ACT_SITE 753 753 Proton acceptor (By similarity).
 BINDING 660 660 ATP (By similarity).
 MOD_RES 609 609 Phosphotyrosine; by autocatalysis (By
 CARBOHYD 343 343 N-linked (GlcNAc...) (Potential).
 CARBOHYD 440 440 N-linked (GlcNAc...) (Potential).
 DISULFID 73 191 By similarity.  
Keyword
 Angiogenesis; ATP-binding; Cell membrane; Cell projection; Complete proteome; Developmental protein; Disulfide bond; Glycoprotein; Kinase; Membrane; Neurogenesis; Nucleotide-binding; Phosphoprotein; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 993 AA 
Protein Sequence
MAGARPPPGL LPLLAPLLLP LLLPAGCWAL EETLMDTKWV TSELAWTSHP ESGWEEVSGY 60
DEAMNPIRTY QVCNVRESSQ NNWLRTGFIW RREVQRVYVE LKFTVRDCNS IPNIPGSCKE 120
TFNLFYYEAD SDVASASSPF WMENPYVKVD TIAPDESFSR LDAGRVNTKV RSFGPLSKAG 180
FYLAFQDQGA CMSLISVRAF YKKCASTTAG FALFPETLTG AEPTSLVIAP GTCIANAVEV 240
SVPLKLYCNG DGEWMVPVGA CTCATGHEPA AKESQCRACP PGSYKAKQGE GPCLPCPPNS 300
RTTSPAASIC TCHNNFYRAD SDSADSACTT VPSPPRGVIS NVNETSLILE WSEPRDLGGR 360
DDLLYNVICK KCRGSSGAGG PATCSRCDDN VEFVPRQLGL TERRVHISHL LAHTRYTFEV 420
QAVNGVSGKS PLPPRYAAVN ITTNQAAPSE VPTLHLHSSS GSSLTLSWAP PERPNGVILD 480
YEMKYFEKSK GIASTVTSQK NSVQLDGLQP DARYVVQVRA RTVAGYGQYS HPAEFETTSE 540
RGSGAQQLQE QLPLIVGSTV AGFVFMVVVV VIALVCLRKQ RHGPDAEYTE KLQQYIAPGM 600
KVYIDPFTYE DPNEAVREFA KEIDVSCVKI EEVIGAGEFG EVCRGRLKLP GRREVFVAIK 660
TLKVGYTERQ RRDFLSEASI MGQFDHPNII RLEGVVTKSR PVMILTEFME NCALDSFLRL 720
NDGQFTVIQL VGMLRGIAAG MKYLSEMNYV HRDLAARNIL VNSNLVCKVS DFGLSRFLED 780
DPSDPTYTSS LGGKIPIRWT APEAIAYRKF TSASDVWSYG IVMWEVMSYG ERPYWDMSNQ 840
DVINAVEQDY RLPPPMDCPT ALHQLMLDCW VRDRNLRPKF SQIVNTLDKL IRNAASLKVT 900
ASAPSGMSQP LLDRTVPDYT TFTTVGDWLD AIKMGRYKES FVGAGFASFD LVAQMTAEDL 960
LRIGVTLAGH QKKILCSIQD MRLQMNQTLP VQV 993 
Gene Ontology
 GO:0030425; C:dendrite; IEA:UniProtKB-SubCell.
 GO:0005887; C:integral to plasma membrane; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008046; F:axon guidance receptor activity; IDA:MGI.
 GO:0005005; F:transmembrane-ephrin receptor activity; TAS:MGI.
 GO:0001525; P:angiogenesis; IMP:UniProtKB.
 GO:0007413; P:axonal fasciculation; IMP:UniProtKB.
 GO:0016477; P:cell migration; IMP:UniProtKB.
 GO:0021952; P:central nervous system projection neuron axonogenesis; IDA:MGI.
 GO:0022038; P:corpus callosum development; IMP:UniProtKB.
 GO:0060997; P:dendritic spine morphogenesis; IMP:UniProtKB.
 GO:0048546; P:digestive tract morphogenesis; IMP:UniProtKB.
 GO:0060021; P:palate development; IMP:UniProtKB.
 GO:0051965; P:positive regulation of synapse assembly; IMP:UniProtKB.
 GO:0046777; P:protein autophosphorylation; ISS:UniProtKB.
 GO:0050770; P:regulation of axonogenesis; IDA:UniProtKB.
 GO:0043088; P:regulation of Cdc42 GTPase activity; ISS:UniProtKB.
 GO:0022407; P:regulation of cell-cell adhesion; ISS:UniProtKB.
 GO:0032314; P:regulation of Rac GTPase activity; ISS:UniProtKB.
 GO:0031290; P:retinal ganglion cell axon guidance; IDA:MGI.
 GO:0034446; P:substrate adhesion-dependent cell spreading; ISS:UniProtKB.
 GO:0048538; P:thymus development; IMP:UniProtKB.
 GO:0001655; P:urogenital system development; IMP:UniProtKB. 
Interpro
 IPR001090; Ephrin_rcpt_lig-bd_dom.
 IPR003961; Fibronectin_type3.
 IPR008979; Galactose-bd-like.
 IPR013783; Ig-like_fold.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR001660; SAM.
 IPR013761; SAM/pointed.
 IPR021129; SAM_type1.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR011641; Tyr-kin_ephrin_A/B_rcpt-like.
 IPR008266; Tyr_kinase_AS.
 IPR020635; Tyr_kinase_cat_dom.
 IPR016257; Tyr_kinase_ephrin_rcpt.
 IPR001426; Tyr_kinase_rcpt_V_CS. 
Pfam
 PF01404; Ephrin_lbd
 PF00041; fn3
 PF07699; GCC2_GCC3
 PF07714; Pkinase_Tyr
 PF00536; SAM_1 
SMART
 SM00615; EPH_lbd
 SM00060; FN3
 SM00454; SAM
 SM00219; TyrKc 
PROSITE
 PS51550; EPH_LBD
 PS50853; FN3
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00109; PROTEIN_KINASE_TYR
 PS00790; RECEPTOR_TYR_KIN_V_1
 PS00791; RECEPTOR_TYR_KIN_V_2
 PS50105; SAM_DOMAIN 
PRINTS
 PR00109; TYRKINASE.