CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013521
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DDB1- and CUL4-associated factor 6 
Protein Synonyms/Alias
 Androgen receptor complex-associated protein; ARCAP; IQ motif and WD repeat-containing protein 1; Nuclear receptor interaction protein; NRIP 
Gene Name
 DCAF6 
Gene Synonyms/Alias
 IQWD1; MSTP055 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
101RANIFSAKFLPCTNDubiquitination[1]
291DDTARELKTPSAEERubiquitination[1]
308ELRQPPVKRLRLRGDubiquitination[1]
652QSDKFTAKPLDSNSGubiquitination[2]
785FRRRKERKEMEELDTubiquitination[1]
891ESRIFNRKLADEVITubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Ligand-dependent coactivator of nuclear receptors. Enhance transcriptional activity of the nuclear receptors NR3C1 and AR. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. 
Sequence Annotation
 REPEAT 49 88 WD 1.
 REPEAT 92 133 WD 2.
 REPEAT 139 179 WD 3.
 REPEAT 189 229 WD 4.
 REPEAT 251 290 WD 5.
 DOMAIN 676 705 IQ.
 REPEAT 718 756 WD 6.
 REPEAT 759 798 WD 7.
 MOD_RES 336 336 Phosphoserine.
 MOD_RES 649 649 Phosphoserine.
 MOD_RES 654 654 Phosphothreonine.
 MOD_RES 657 657 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Ubl conjugation pathway; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 860 AA 
Protein Sequence
MSRGGSYPHL LWDVRKRSLG LEDPSRLRSR YLGRREFIQR LKLEATLNVH DGCVNTICWN 60
DTGEYILSGS DDTKLVISNP YSRKVLTTIR SGHRANIFSA KFLPCTNDKQ IVSCSGDGVI 120
FYTNVEQDAE TNRQCQFTCH YGTTYEIMTV PNDPYTFLSC GEDGTVRWFD TRIKTSCTKE 180
DCKDDILINC RRAATSVAIC PPIPYYLAVG CSDSSVRIYD RRMLGTRATG NYAGRGTTGM 240
VARFIPSHLN NKSCRVTSLC YSEDGQEILV SYSSDYIYLF DPKDDTAREL KTPSAEERRE 300
ELRQPPVKRL RLRGDWSDTG PRARPESERE RDGEQSPNVS LMQRMSDMLS RWFEEASEVA 360
QSNRGRGRSR PRGGTSQSDI STLPTVPSSP DLEVSETAME VDTPAEQFLQ PSTSSTMSAQ 420
AHSTSSPTES PHSTPLLSSP DSEQRQSVEA SGHHTHHQSE FLRGPEIALL RKRLQQLRLK 480
KAEQQRQQEL AAHTQQQPST SDQSSHEGSS QDPHASDSPS SVVNKQLGSM SLDEQQDNNN 540
EKLSPKPGTG EPVLSLHYST EGTTTSTIKL NFTDEWSSIA SSSRGIGSHC KSEGQEESFV 600
PQSSVQPPEG DSETKAPEES SEDVTKYQEG VSAENPVENH INITQSDKFT AKPLDSNSGE 660
RNDLNLDRSC GVPEESASSE KAKEPETSDQ TSTESATNEN NTNPEPQFQT EATGPSAHEE 720
TSTRDSALQD TDDSDDDPVL IPGARYRAGP GDRFNIRGTT IGDRIMRRSA VARIQEFFRR 780
RKERKEMEEL DTLNIRRPLV KMVYKGHRNS RTMIKEANFW GANFVMSGSD CGHIFIWDRH 840
TAEHLMLLEA DNHVVNCLQP HPFDPILASS GIDYDIKIWS PLEESRIFNR KLADEVITRN 900
ELMLEETRNT ITVPASFMLR MLASLNHIRA DRLEGDRSEG SGQENENEDE E 951 
Gene Ontology
 GO:0080008; C:Cul4-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0030374; F:ligand-dependent nuclear receptor transcription coactivator activity; IDA:MGI.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IGI:MGI.
 GO:0016567; P:protein ubiquitination; IC:UniProtKB. 
Interpro
 IPR000048; IQ_motif_EF-hand-BS.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00015; IQ
 SM00320; WD40 
PROSITE
 PS50096; IQ
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS