UniProt Accession

 ZEO1_YEAST; Q08245; D6W1V8 

Genbank Protein ID

 Z74851; AY693207; BK006948 

Genbank Nucleotide ID

 CAA99128.1; AAT93226.1; DAA10674.1 

Protein Name

 Protein ZEO1 

Protein Synonyms/Alias

 Zeocin resistance protein 1 

Gene Name

 ZEO1 

Gene Synonyms/Alias

 YOL109W; O0738 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
7	*MSEIQNKAETAAQD	acetylation	[1]
18	AAQDVQQKLEETKES	acetylation	[1]
18	AAQDVQQKLEETKES	ubiquitination	[2, 3, 4]
23	QQKLEETKESLQNKG	acetylation	[1]
23	QQKLEETKESLQNKG	ubiquitination	[2, 3, 4]
29	TKESLQNKGQEVKEQ	acetylation	[1]
29	TKESLQNKGQEVKEQ	ubiquitination	[3, 4]
34	QNKGQEVKEQAEASI	acetylation	[1]
34	QNKGQEVKEQAEASI	ubiquitination	[3, 4]
45	EASIDNLKNEATPEA	acetylation	[1]
45	EASIDNLKNEATPEA	ubiquitination	[2, 3, 4, 5, 6, 7]
56	TPEAEQVKKEEQNIA	acetylation	[1]
56	TPEAEQVKKEEQNIA	ubiquitination	[3, 4]
57	PEAEQVKKEEQNIAD	acetylation	[1]
57	PEAEQVKKEEQNIAD	ubiquitination	[2, 3, 4, 5, 6, 7]
69	IADGVEQKKTEAANK	ubiquitination	[3, 4]
76	KKTEAANKVEETKKQ	acetylation	[1]
76	KKTEAANKVEETKKQ	ubiquitination	[4]
81	ANKVEETKKQASAAV	ubiquitination	[3]
82	NKVEETKKQASAAVS	ubiquitination	[3, 4]
91	ASAAVSEKKETKKEG	ubiquitination	[3, 4]
107	FLKKLNRKIASIFN*	ubiquitination	[4]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis.
 Ziv I, Matiuhin Y, Kirkpatrick DS, Erpapazoglou Z, Leon S, Pantazopoulou M, Kim W, Gygi SP, Haguenauer-Tsapis R, Reis N, Glickman MH, Kleifeld O.
 Mol Cell Proteomics. 2011 May;10(5):M111.009753. [PMID: 21427232]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [4] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [5] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [6] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [7] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 Acts antagonistically to MID2 in signaling cell wall stress to the PKC1-MPK1 cell integrity pathway. 

Sequence Annotation

 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine.
 MOD_RES 25 25 Phosphoserine.
 MOD_RES 40 40 Phosphoserine.
 MOD_RES 49 49 Phosphothreonine.
 CROSSLNK 45 45 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 57 57 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Cell membrane; Coiled coil; Complete proteome; Direct protein sequencing; Isopeptide bond; Membrane; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 113 AA 

Protein Sequence

Gene Ontology

 GO:0019897; C:extrinsic to plasma membrane; IDA:SGD.
 GO:0005741; C:mitochondrial outer membrane; IDA:SGD.
 GO:0031505; P:fungal-type cell wall organization; IGI:SGD. 

Interpro

  

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SMART

  

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PRINTS