CPLM-001647

Genbank Nucleotide ID

Geranylgeranyl pyrophosphate synthase

Protein Synonyms/Alias

GGPP synthase; GGPPSase; (2E,6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; Farnesyltranstransferase; Geranylgeranyl diphosphate synthase; Geranyltranstransferase

Homo sapiens (Human)

Position	Peptide	Type	References
3	*****MEKTQETVQR	ubiquitination	[1, 2]
17	RILLEPYKYLLQLPG	ubiquitination	[2, 3, 4]
25	YLLQLPGKQVRTKLS	acetylation	[5]
113	LDHPDAVKLFTRQLL	ubiquitination	[3, 4]
196	DYANLHSKEYSENKS	ubiquitination	[6]
273	ELEAKAYKQIDARGG	ubiquitination	[2]

[1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]

Functional Description

Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate, an important precursor of carotenoids and geranylated proteins.

METAL 64 64 Magnesium 1.
METAL 64 64 Magnesium 2.
METAL 68 68 Magnesium 1.
METAL 68 68 Magnesium 2.
METAL 188 188 Magnesium 3 (By similarity).
BINDING 25 25 Isopentenyl diphosphate (By similarity).
BINDING 28 28 Isopentenyl diphosphate (By similarity).
BINDING 57 57 Isopentenyl diphosphate (By similarity).
BINDING 73 73 Dimethylallyl diphosphate.
BINDING 74 74 Isopentenyl diphosphate (By similarity).
BINDING 151 151 Dimethylallyl diphosphate.
BINDING 152 152 Dimethylallyl diphosphate.
BINDING 185 185 Dimethylallyl diphosphate.
BINDING 202 202 Dimethylallyl diphosphate.
BINDING 212 212 Dimethylallyl diphosphate (By

3D-structure; Complete proteome; Cytoplasm; Isoprene biosynthesis; Magnesium; Metal-binding; Reference proteome; Transferase.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0004161; F:dimethylallyltranstransferase activity; IEA:EC.
GO:0004311; F:farnesyltranstransferase activity; IDA:UniProtKB.
GO:0004337; F:geranyltranstransferase activity; IEA:EC.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0006695; P:cholesterol biosynthetic process; TAS:Reactome.
GO:0045337; P:farnesyl diphosphate biosynthetic process; IEA:UniProtKB-UniPathway.
GO:0033384; P:geranyl diphosphate biosynthetic process; IEA:UniProtKB-UniPathway.
GO:0033386; P:geranylgeranyl diphosphate biosynthetic process; IEA:UniProtKB-UniPathway.
GO:0006720; P:isoprenoid metabolic process; IDA:UniProtKB.

IPR000092; Polyprenyl_synt.
IPR017446; Polyprenyl_synth-rel.
IPR008949; Terpenoid_synth.

PF00348; polyprenyl_synt

PS00723; POLYPRENYL_SYNTHASE_1
PS00444; POLYPRENYL_SYNTHASE_2