CPLM-020251

Genbank Nucleotide ID

Guanine nucleotide-binding protein-like 3

Protein Synonyms/Alias

E2-induced gene 3 protein; Novel nucleolar protein 47; NNP47; Nucleolar GTP-binding protein 3; Nucleostemin

Homo sapiens (Human)

Position	Peptide	Type	References
57	VPNSAPFKEALLREA	ubiquitination	[1, 2]
79	EELKQQQKLDRQKEL	acetylation	[3]
136	LYCQELKKVIEASDV	ubiquitination	[2]
171	AIVQSGQKKLVLILN	ubiquitination	[4]
179	KLVLILNKSDLVPKE	acetylation	[5]
179	KLVLILNKSDLVPKE	ubiquitination	[2]
196	ESWLNYLKKELPTVV	ubiquitination	[6]
237	RSEVCFGKEGLWKLL	ubiquitination	[2, 6]
253	GFQETCSKAIRVGVI	ubiquitination	[2, 7]
332	PASIEVVKPMEAASA	ubiquitination	[6]
352	DARQVVLKYTVPGYR	ubiquitination	[2]

[1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[5] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
[6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).

DOMAIN 266 347 G.
NP_BIND 178 181 GTP (Potential).
NP_BIND 261 268 GTP (Potential).
NP_BIND 305 308 GTP (Potential).
REGION 2 46 Basic (By similarity).
REGION 282 456 Intermediate (By similarity).
REGION 465 543 Acidic (By similarity).
MOD_RES 79 79 N6-acetyllysine.
MOD_RES 529 529 Phosphoserine.

Acetylation; Alternative splicing; Coiled coil; Complete proteome; GTP-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005730; C:nucleolus; ISS:UniProtKB.
GO:0005654; C:nucleoplasm; IEA:Compara.
GO:0005525; F:GTP binding; ISS:UniProtKB.
GO:0003924; F:GTPase activity; IBA:RefGenome.
GO:0008283; P:cell proliferation; IEA:Compara.
GO:0042127; P:regulation of cell proliferation; ISS:UniProtKB.
GO:0042254; P:ribosome biogenesis; IBA:RefGenome.

IPR014813; Gnl3_N_dom.
IPR006073; GTP_binding_domain.
IPR027417; P-loop_NTPase.

PF08701; GN3L_Grn1
PF01926; MMR_HSR1