CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005752
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tripeptidyl-peptidase 2 
Protein Synonyms/Alias
 TPP-2; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase II; TPP-II 
Gene Name
 TPP2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
19FHGLLPKKETGAASFubiquitination[1, 2, 3]
61MQVTTDGKPKIVDIIubiquitination[4]
109SWTNPSGKYHIGIKNubiquitination[2, 3]
152ALAEACRKQEEFDVAubiquitination[2, 3]
220NEDGDLSKSTVLRNYubiquitination[2, 3]
321MIEVINHKCDLVNYSubiquitination[2]
401AEYSLREKLPANQYTubiquitination[2, 3]
655AFTDVHFKPGQIRRHubiquitination[2]
697LHAVQLVKQRAYRSHubiquitination[2]
708YRSHEFYKFCSLPEKacetylation[3, 5]
708YRSHEFYKFCSLPEKubiquitination[2, 3]
715KFCSLPEKGTLTEAFubiquitination[2]
880YPHQYSLKLEKGDYTacetylation[5, 6]
883QYSLKLEKGDYTIRLacetylation[3]
883QYSLKLEKGDYTIRLubiquitination[3]
904ISDLERLKDLPFIVSubiquitination[2]
958VTSLPDDKIPKGAGPacetylation[6]
958VTSLPDDKIPKGAGPubiquitination[2, 3]
1005TKTKNGSKDKEKDSEubiquitination[7, 8]
1013DKEKDSEKEKDLKEEubiquitination[7, 8]
1029TEALRDLKIQWMTKLubiquitination[2]
1067LHQLDAEKERMKRLNubiquitination[2]
1180WTDLFDNKVLTFAYKubiquitination[2, 8, 9]
1187KVLTFAYKHALVNKMubiquitination[2, 8, 9]
1193YKHALVNKMYGRGLKubiquitination[2, 3]
1200KMYGRGLKFATKLVEubiquitination[2]
1204RGLKFATKLVEEKPTacetylation[3]
1204RGLKFATKLVEEKPTubiquitination[2, 3]
1212LVEEKPTKENWKNCIubiquitination[2, 3]
1216KPTKENWKNCIQLMKubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [7] Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
 Denis NJ, Vasilescu J, Lambert JP, Smith JC, Figeys D.
 Proteomics. 2007 Mar;7(6):868-74. [PMID: 17370265]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [9] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. May be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited (By similarity). 
Sequence Annotation
 ACT_SITE 44 44 Charge relay system (By similarity).
 ACT_SITE 264 264 Charge relay system (By similarity).
 ACT_SITE 449 449 Charge relay system (By similarity).
 MOD_RES 2 2 N-acetylalanine.
 CROSSLNK 1005 1005 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 1013 1013 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Aminopeptidase; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Isopeptide bond; Nucleus; Protease; Reference proteome; Serine protease; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1249 AA 
Protein Sequence
MATAATEEPF PFHGLLPKKE TGAASFLCRY PEYDGRGVLI AVLDTGVDPG APGMQVTTDG 60
KPKIVDIIDT TGSGDVNTAT EVEPKDGEIV GLSGRVLKIP ASWTNPSGKY HIGIKNGYDF 120
YPKALKERIQ KERKEKIWDP VHRVALAEAC RKQEEFDVAN NGSSQANKLI KEELQSQVEL 180
LNSFEKKYSD PGPVYDCLVW HDGEVWRACI DSNEDGDLSK STVLRNYKEA QEYGSFGTAE 240
MLNYSVNIYD DGNLLSIVTS GGAHGTHVAS IAAGHFPEEP ERNGVAPGAQ ILSIKIGDTR 300
LSTMETGTGL IRAMIEVINH KCDLVNYSYG EATHWPNSGR ICEVINEAVW KHNIIYVSSA 360
GNNGPCLSTV GCPGGTTSSV IGVGAYVSPD MMVAEYSLRE KLPANQYTWS SRGPSADGAL 420
GVSISAPGGA IASVPNWTLR GTQLMNGTSM SSPNACGGIA LILSGLKANN IDYTVHSVRR 480
ALENTAVKAD NIEVFAQGHG IIQVDKAYDY LVQNTSFANK LGFTVTVGNN RGIYLRDPVQ 540
VAAPSDHGVG IEPVFPENTE NSEKISLQLH LALTSNSSWV QCPSHLELMN QCRHINIRVD 600
PRGLREGLHY TEVCGYDIAS PNAGPLFRVP ITAVIAAKVN ESSHYDLAFT DVHFKPGQIR 660
RHFIEVPEGA TWAEVTVCSC SSEVSAKFVL HAVQLVKQRA YRSHEFYKFC SLPEKGTLTE 720
AFPVLGGKAI EFCIARWWAS LSDVNIDYTI SFHGIVCTAP QLNIHASEGI NRFDVQSSLK 780
YEDLAPCITL KNWVQTLRPV SAKTKPLGSR DVLPNNRQLY EMVLTYNFHQ PKSGEVTPSC 840
PLLCELLYES EFDSQLWIIF DQNKRQMGSG DAYPHQYSLK LEKGDYTIRL QIRHEQISDL 900
ERLKDLPFIV SHRLSNTLSL DIHENHSFAL LGKKKSSNLT LPPKYNQPFF VTSLPDDKIP 960
KGAGPGCYLA GSLTLSKTEL GKKADVIPVH YYLIPPPTKT KNGSKDKEKD SEKEKDLKEE 1020
FTEALRDLKI QWMTKLDSSD IYNELKETYP NYLPLYVARL HQLDAEKERM KRLNEIVDAA 1080
NAVISHIDQT ALAVYIAMKT DPRPDAATIK NDMDKQKSTL VDALCRKGCA LADHLLHTQA 1140
QDGAISTDAE GKEEEGESPL DSLAETFWET TKWTDLFDNK VLTFAYKHAL VNKMYGRGLK 1200
FATKLVEEKP TKENWKNCIQ LMKLLGWTHC ASFTENWLPI MYPPDYCVF 1249 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
 GO:0004252; F:serine-type endopeptidase activity; IBA:RefGenome.
 GO:0008240; F:tripeptidyl-peptidase activity; TAS:ProtInc.
 GO:0002474; P:antigen processing and presentation of peptide antigen via MHC class I; TAS:Reactome.
 GO:0000209; P:protein polyubiquitination; TAS:Reactome.
 GO:0006508; P:proteolysis; IBA:RefGenome. 
Interpro
 IPR000209; Peptidase_S8/S53_dom.
 IPR022398; Peptidase_S8_His-AS.
 IPR023828; Peptidase_S8_Ser-AS.
 IPR015500; Peptidase_S8_subtilisin-rel.
 IPR022229; Peptidase_S8A_TPPII. 
Pfam
 PF00082; Peptidase_S8
 PF12580; TPPII 
SMART
  
PROSITE
 PS00136; SUBTILASE_ASP
 PS00137; SUBTILASE_HIS
 PS00138; SUBTILASE_SER 
PRINTS
 PR00723; SUBTILISIN.