UniProt Accession

 ACBD6_HUMAN; Q9BR61 

Genbank Protein ID

 AL445469; AL139141; AL358354; AL139141; AL358354; AL445469; AL358354; AL139141; AL445469; BC006505 

Genbank Nucleotide ID

 CAH71747.1; CAH71747.1; CAH71747.1; CAI19365.1; CAI19365.1; CAI19365.1; CAI15093.1; CAI15093.1; CAI15093.1; AAH06505.1 

Protein Name

 Acyl-CoA-binding domain-containing protein 6 

Protein Synonyms/Alias

  

Gene Name

 ACBD6 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
95	FDFEGKQKWEAWKAL	ubiquitination	[1]
176	NNIDHITKAIKSKNV	ubiquitination	[2, 3, 4]
179	DHITKAIKSKNVDVN	ubiquitination	[2, 3]
181	ITKAIKSKNVDVNVK	ubiquitination	[1]
268	PEEVTGCKTVSLVLQ	ubiquitination	[5, 6]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 

Functional Description

 Binds long-chain acyl-coenzyme A molecules with a strong preference for unsaturated C18:1-CoA, lower affinity for unsaturated C20:4-CoA, and very weak affinity for saturated C16:0- CoA. Does not bind fatty acids. 

Sequence Annotation

 DOMAIN 42 127 ACB.
 REPEAT 191 220 ANK 1.
 REPEAT 224 253 ANK 2.
 REGION 69 73 Acyl-CoA binding (By similarity).
 BINDING 95 95 Acyl-CoA (By similarity).
 BINDING 114 114 Acyl-CoA (By similarity).
 MOD_RES 69 69 Phosphotyrosine (By similarity).
 MOD_RES 106 106 Phosphoserine.  

Keyword

 3D-structure; ANK repeat; Complete proteome; Cytoplasm; Lipid-binding; Phosphoprotein; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 282 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0000062; F:fatty-acyl-CoA binding; IEA:InterPro.
 GO:0008289; F:lipid binding; IEA:UniProtKB-KW. 

Interpro

 IPR000582; Acyl-CoA-binding_protein.
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR014352; FERM/acyl-CoA-bd_prot_3-hlx. 

Pfam

 PF00887; ACBP
 PF12796; Ank_2 

SMART

 SM00248; ANK 

PROSITE

 PS00880; ACB_1
 PS51228; ACB_2
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 

PRINTS

 PR00689; ACOABINDINGP.
 PR01415; ANKYRIN.