CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022929
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Signal recognition particle subunit SRP68 
Protein Synonyms/Alias
 SRP68; Signal recognition particle 68 kDa protein 
Gene Name
 SRP68 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
39GAGGEENKENERPSAubiquitination[1]
49ERPSAGSKANKEFGDubiquitination[1]
112RHKFTGKKVTEELLTubiquitination[1]
142WSYAMQLKQEANTEPubiquitination[1, 2, 3, 4]
205RFEHQEWKAAIEAFNubiquitination[2, 3, 4]
220KCKTIYEKLASAFTEubiquitination[1, 2, 3, 4, 5]
277TEGLLAEKLEALITQubiquitination[1, 2, 3, 4, 5]
288LITQTRAKQAATMSEubiquitination[1, 3]
333QAESEETKERLFESMubiquitination[1, 3, 6]
360EELKPDQKQRDYILEubiquitination[1]
372ILEGEPGKVSNLQYLubiquitination[1]
400KRNENMAKGLQRALLubiquitination[3]
416QQPEDDSKRSPRPQDubiquitination[1, 3]
452EEDKAFQKEIGLKTLacetylation[7]
452EEDKAFQKEIGLKTLubiquitination[1]
457FQKEIGLKTLVFKAYubiquitination[1, 5]
477AQSYVLVKKWSEALVubiquitination[1]
491VLYDRVLKYANEVNSubiquitination[1]
504NSDAGAFKNSLKDLPubiquitination[1, 2, 3, 4]
508GAFKNSLKDLPDVQEubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP68 binds the 7S RNA, SRP72 binds to this complex subsequently. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function. 
Sequence Annotation
 MOD_RES 48 48 Phosphoserine.
 MOD_RES 452 452 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome; Ribonucleoprotein; RNA-binding; Signal recognition particle. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 627 AA 
Protein Sequence
MAAEKQVPGG GGGGGSGGGG GSGGGGSGGG RGAGGEENKE NERPSAGSKA NKEFGDSLSL 60
EILQIIKESQ QQHGLRHGDF QRYRGYCSRR QRRLRKTLNF KMGNRHKFTG KKVTEELLTD 120
NRYLLLVLMD AERAWSYAMQ LKQEANTEPR KRFHLLSRLR KAVKHAEELE RLCESNRVDA 180
KTKLEAQAYT AYLSGMLRFE HQEWKAAIEA FNKCKTIYEK LASAFTEEQA VLYNQRVEEI 240
SPNIRYCAYN IGDQSAINEL MQMRLRSGGT EGLLAEKLEA LITQTRAKQA ATMSEVEWRG 300
RTVPVKIDKV RIFLLGLADN EAAIVQAESE ETKERLFESM LSECRDAIQV VREELKPDQK 360
QRDYILEGEP GKVSNLQYLH SYLTYIKLST AIKRNENMAK GLQRALLQQQ PEDDSKRSPR 420
PQDLIRLYDI ILQNLVELLQ LPGLEEDKAF QKEIGLKTLV FKAYRCFFIA QSYVLVKKWS 480
EALVLYDRVL KYANEVNSDA GAFKNSLKDL PDVQELITQV RSEKCSLQAA AILDANDAHQ 540
TETSSSQVKD NKPLVERFET FCLDPSLVTK QANLVHFPPG FQPIPCKPLF FDLALNHVAF 600
PPLEDKLEQK TKSGLTGYIK GIFGFRS 627 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005783; C:endoplasmic reticulum; TAS:ProtInc.
 GO:0005730; C:nucleolus; TAS:ProtInc.
 GO:0005840; C:ribosome; TAS:ProtInc.
 GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; IDA:UniProtKB.
 GO:0008312; F:7S RNA binding; IEA:InterPro.
 GO:0030942; F:endoplasmic reticulum signal peptide binding; IEA:InterPro.
 GO:0005047; F:signal recognition particle binding; IPI:UniProtKB.
 GO:0042493; P:response to drug; IDA:UniProtKB.
 GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
 GO:0006412; P:translation; TAS:Reactome. 
Interpro
 IPR026258; SRP68. 
Pfam
  
SMART
  
PROSITE
  
PRINTS