CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000299
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transportin-2 
Protein Synonyms/Alias
 Karyopherin beta-2b 
Gene Name
 TNPO2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
71SLSGLILKNNVKAHYubiquitination[1]
334PDSEQDIKPRFHKSRubiquitination[1]
817NIRDNEEKDSAFRGIubiquitination[2, 3]
862DLRDMFYKILHGFKDacetylation[4]
862DLRDMFYKILHGFKDubiquitination[5]
888EQFPPLLKERLAAFYubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Probably functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). 
Sequence Annotation
 DOMAIN 31 99 Importin N-terminal.
 REPEAT 121 159 HEAT 1.
 REPEAT 167 205 HEAT 2.
 REPEAT 208 246 HEAT 3.
 REPEAT 249 287 HEAT 4.
 REPEAT 292 330 HEAT 5.
 REPEAT 389 427 HEAT 6.
 REPEAT 430 468 HEAT 7.
 REPEAT 472 510 HEAT 8.
 REPEAT 513 551 HEAT 9.
 REPEAT 556 596 HEAT 10.
 REPEAT 659 697 HEAT 11.
 REPEAT 700 738 HEAT 12.
 REPEAT 741 780 HEAT 13.
 MOD_RES 862 862 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Nucleus; Polymorphism; Protein transport; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 897 AA 
Protein Sequence
MDWQPDEQGL QQVLQLLKDS QSPNTATQRI VQDKLKQLNQ FPDFNNYLIF VLTRLKSEDE 60
PTRSLSGLIL KNNVKAHYQS FPPPVADFIK QECLNNIGDA SSLIRATIGI LITTIASKGE 120
LQMWPELLPQ LCNLLNSEDY NTCEGAFGAL QKICEDSSEL LDSDALNRPL NIMIPKFLQF 180
FKHCSPKIRS HAIACVNQFI MDRAQALMDN IDTFIEHLFA LAVDDDPEVR KNVCRALVML 240
LEVRIDRLIP HMHSIIQYML QRTQDHDENV ALEACEFWLT LAEQPICKEV LASHLVQLIP 300
ILVNGMKYSE IDIILLKGDV EEDEAVPDSE QDIKPRFHKS RTVTLPHEAE RPDGSEDAED 360
DDDDDALSDW NLRKCSAAAL DVLANVFREE LLPHLLPLLK GLLFHPEWVV KESGILVLGA 420
IAEGCMQGMV PYLPELIPHL IQCLSDKKAL VRSIACWTLS RYAHWVVSQP PDMHLKPLMT 480
ELLKRILDGN KRVQEAACSA FATLEEEACT ELVPYLSYIL DTLVFAFGKY QHKNLLILYD 540
AIGTLADSVG HHLNQPEYIQ KLMPPLIQKW NELKDEDKDL FPLLECLSSV ATALQSGFLP 600
YCEPVYQRCV TLVQKTLAQA MMYTQHPEQY EAPDKDFMIV ALDLLSGLAE GLGGHVEQLV 660
ARSNIMTLLF QCMQDSMPEV RQSSFALLGD LTKACFIHVK PCIAEFMPIL GTNLNPEFIS 720
VCNNATWAIG EICMQMGAEM QPYVQMVLNN LVEIINRPNT PKTLLENTGR LTSPSAIPAI 780
TIGRLGYVCP QEVAPMLQQF IRPWCTSLRN IRDNEEKDSA FRGICMMIGV NPGGVVQDFI 840
FFCDAVASWV SPKDDLRDMF YKILHGFKDQ VGEDNWQQFS EQFPPLLKER LAAFYGV 897 
Gene Ontology
 GO:0005737; C:cytoplasm; TAS:ProtInc.
 GO:0005643; C:nuclear pore; IEA:InterPro.
 GO:0005634; C:nucleus; TAS:ProtInc.
 GO:0008139; F:nuclear localization sequence binding; TAS:ProtInc.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IEA:InterPro. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000357; HEAT.
 IPR001494; Importin-beta_N. 
Pfam
 PF02985; HEAT
 PF03810; IBN_N 
SMART
 SM00913; IBN_N 
PROSITE
 PS50077; HEAT_REPEAT
 PS50166; IMPORTIN_B_NT 
PRINTS