CPLM-001104

Genbank Nucleotide ID

WD repeat-containing protein 1

Protein Synonyms/Alias

Actin-interacting protein 1; AIP1; NORI-1

Homo sapiens (Human)

Position	Peptide	Type	References
7	*MPYEIKKVFASLPQ	ubiquitination	[1, 2]
21	QVERGVSKIIGGDPK	ubiquitination	[2, 3, 4]
28	KIIGGDPKGNNFLYT	acetylation	[5]
28	KIIGGDPKGNNFLYT	ubiquitination	[1, 2]
38	NFLYTNGKCVILRNI	ubiquitination	[1, 2, 3]
81	ASGDVSGKLRIWDTT	acetylation	[5]
81	ASGDVSGKLRIWDTT	ubiquitination	[2]
90	RIWDTTQKEHLLKYE	ubiquitination	[1, 2, 3]
95	TQKEHLLKYEYQPFA	acetylation	[5]
95	TQKEHLLKYEYQPFA	ubiquitination	[1, 2, 4]
104	EYQPFAGKIKDIAWT	ubiquitination	[2, 3, 4]
106	QPFAGKIKDIAWTED	ubiquitination	[2, 3]
115	IAWTEDSKRIAVVGE	acetylation	[5]
115	IAWTEDSKRIAVVGE	ubiquitination	[2, 3]
126	VVGEGREKFGAVFLW	ubiquitination	[2]
155	VINSVDIKQSRPYRL	ubiquitination	[2, 3, 4]
180	FFEGPPFKFKFTIGD	ubiquitination	[2, 3]
182	EGPPFKFKFTIGDHS	ubiquitination	[2]
219	QIYIYDGKTGEKVCA	ubiquitination	[2, 4]
223	YDGKTGEKVCALGGS	ubiquitination	[2, 3]
231	VCALGGSKAHDGGIY	ubiquitination	[2]
256	LLSASGDKTSKIWDV	acetylation	[6, 7]
256	LLSASGDKTSKIWDV	ubiquitination	[8]
311	LDRNNPSKPLHVIKG	ubiquitination	[2, 7]
317	SKPLHVIKGHSKSIQ	ubiquitination	[2]
321	HVIKGHSKSIQCLTV	acetylation	[7]
321	HVIKGHSKSIQCLTV	ubiquitination	[2, 7]
334	TVHKNGGKSYIYSGS	ubiquitination	[2, 3, 7]
362	ENDSFAGKGHTNQVS	ubiquitination	[2, 4, 8, 9]
405	YSGQGVVKLDVQPKC	ubiquitination	[1, 2, 3, 7, 8]
437	VLLKDQRKCFSIDNP	ubiquitination	[2, 3, 7]
480	SILGTTLKDEGKLLE	acetylation	[5, 7]
480	SILGTTLKDEGKLLE	ubiquitination	[2, 3, 4, 7, 8, 9]
484	TTLKDEGKLLEAKGP	ubiquitination	[2, 7, 8]
489	EGKLLEAKGPVTDVA	ubiquitination	[2, 3]
511	LAVCDASKVVTVFSV	acetylation	[5]
569	SDPETRVKIQDAHRL	acetylation	[7]
569	SDPETRVKIQDAHRL	ubiquitination	[2, 3]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[8] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[9] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]

Functional Description

Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins (By similarity).

REPEAT 56 95 WD 1.
REPEAT 100 143 WD 2.
REPEAT 144 184 WD 3.
REPEAT 187 226 WD 4.
REPEAT 232 271 WD 5.
REPEAT 318 359 WD 6.
REPEAT 363 401 WD 7.
REPEAT 443 482 WD 8.
REPEAT 487 526 WD 9.
REPEAT 530 569 WD 10.
REPEAT 574 605 WD 11.
MOD_RES 28 28 N6-acetyllysine.
MOD_RES 81 81 N6-acetyllysine.
MOD_RES 95 95 N6-acetyllysine.
MOD_RES 115 115 N6-acetyllysine.
MOD_RES 238 238 Phosphotyrosine.
MOD_RES 480 480 N6-acetyllysine.

Acetylation; Actin-binding; Alternative splicing; Cell junction; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Phosphoprotein; Polymorphism; Reference proteome; Repeat; WD repeat.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0030054; C:cell junction; IEA:UniProtKB-KW.
GO:0042995; C:cell projection; IEA:UniProtKB-KW.
GO:0005829; C:cytosol; TAS:Reactome.
GO:0005576; C:extracellular region; TAS:Reactome.
GO:0002102; C:podosome; IEA:UniProtKB-SubCell.
GO:0030168; P:platelet activation; TAS:Reactome.
GO:0002576; P:platelet degranulation; TAS:Reactome.
GO:0007605; P:sensory perception of sound; TAS:ProtInc.

IPR020472; G-protein_beta_WD-40_rep.
IPR015943; WD40/YVTN_repeat-like_dom.
IPR001680; WD40_repeat.
IPR019775; WD40_repeat_CS.
IPR017986; WD40_repeat_dom.

PS00678; WD_REPEATS_1
PS50082; WD_REPEATS_2
PS50294; WD_REPEATS_REGION

PR00320; GPROTEINBRPT.