CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013013
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription activator BRG1 
Protein Synonyms/Alias
 ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Protein brahma homolog 1; SNF2-beta; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 
Gene Name
 Smarca4 
Gene Synonyms/Alias
 Baf190a; Brg1; Snf2b; Snf2l4 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
1484KERIRNHKYRSLNDLubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron- specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post- mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1 (By similarity). 
Sequence Annotation
 DOMAIN 171 206 QLQ.
 DOMAIN 460 532 HSA.
 DOMAIN 766 931 Helicase ATP-binding.
 DOMAIN 1084 1246 Helicase C-terminal.
 DOMAIN 1443 1513 Bromo.
 NP_BIND 779 786 ATP (Potential).
 REGION 1 282 Necessary for interaction with
 MOTIF 881 884 DEGH box (By similarity).
 MOD_RES 11 11 Phosphothreonine (By similarity).
 MOD_RES 188 188 N6-acetyllysine (By similarity).
 MOD_RES 353 353 Phosphothreonine (By similarity).
 MOD_RES 609 609 Phosphothreonine (By similarity).
 MOD_RES 610 610 Phosphoserine.
 MOD_RES 613 613 Phosphoserine.
 MOD_RES 695 695 Phosphoserine.
 MOD_RES 699 699 Phosphoserine (By similarity).
 MOD_RES 1349 1349 Phosphoserine (By similarity).
 MOD_RES 1419 1419 Phosphoserine.
 MOD_RES 1536 1536 Phosphoserine (By similarity).
 MOD_RES 1541 1541 Phosphoserine (By similarity).
 MOD_RES 1552 1552 Phosphoserine (By similarity).
 MOD_RES 1593 1593 Phosphoserine (By similarity).
 MOD_RES 1597 1597 Phosphoserine (By similarity).  
Keyword
 Acetylation; Activator; Alternative splicing; ATP-binding; Bromodomain; Chromatin regulator; Complete proteome; Helicase; Hydrolase; Neurogenesis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1613 AA 
Protein Sequence
MSTPDPPLGG TPRPGPSPGP GPSPGAMLGP SPGPSPGSAH SMMGPSPGPP SAGHPMPTQG 60
PGGYPQDNMH QMHKPMESMH EKGMPDDPRY NQMKGMGMRS GAHTGMAPPP SPMDQHSQGY 120
PSPLGGSEHA SSPVPASGPS SGPQMSSGPG GAPLDGSDPQ ALGQQNRGPT PFNQNQLHQL 180
RAQIMAYKML ARGQPLPDHL QMAVQGKRPM PGMQQQMPTL PPPSVSATGP GPGPGPGPGP 240
GPGPAPPNYS RPHGMGGPNM PPPGPSGVPP GMPGQPPGGP PKPWPEGPMA NAAAPTSTPQ 300
KLIPPQPTGR PSPAPPAVPP AASPVMPPQT QSPGQPAQPA PLVPLHQKQS RITPIQKPRG 360
LDPVEILQER EYRLQARIAH RIQELENLPG SLAGDLRTKA TIELKALRLL NFQRQLRQEV 420
VVCMRRDTAL ETALNAKAYK RSKRQSLREA RITEKLEKQQ KIEQERKRRQ KHQEYLNSIL 480
QHAKDFREYH RSVTGKLQKL TKAVATYHAN TEREQKKENE RIEKERMRRL MAEDEEGYRK 540
LIDQKKDKRL AYLLQQTDEY VANLTELVRQ HKAAQVAKEK KKKKKKKKAE NAEGQTPAIG 600
PDGEPLDETS QMSDLPVKVI HVESGKILTG TDAPKAGQLE AWLEMNPGYE VAPRSDSEES 660
GSEEEEEEEE EEQPQPAQPP TLPVEEKKKI PDPDSDDVSE VDARHIIENA KQDVDDEYGV 720
SQALARGLQS YYAVAHAVTE RVDKQSALMV NGVLKQYQIK GLEWLVSLYN NNLNGILADE 780
MGLGKTIQTI ALITYLMEHK RINGPFLIIV PLSTLSNWAY EFDKWAPSVV KVSYKGSPAA 840
RRAFVPQLRS GKFNVLLTTY EYIIKDKHIL AKIRWKYMIV DEGHRMKNHH CKLTQVLNTH 900
YVAPRRLLLT GTPLQNKLPE LWALLNFLLP TIFKSCSTFE QWFNAPFAMT GEKVDLNEEE 960
TILIIRRLHK VLRPFLLRRL KKEVEAQLPE KVEYVIKCDM SALQRVLYRH MQAKGVLLTD 1020
GSEKDKKGKG GTKTLMNTIM QLRKICNHPY MFQHIEESFS EHLGFTGGIV QGLDLYRASG 1080
KFELLDRILP KLRATNHKVL LFCQMTSLMT IMEDYFAYRG FKYLRLDGTT KAEDRGMLLK 1140
TFNEPGSEYF IFLLSTRAGG LGLNLQSADT VIIFDSDWNP HQDLQAQDRA HRIGQQNEVR 1200
VLRLCTVNSV EEKILAAAKY KLNVDQKVIQ AGMFDQKSSS HERRAFLQAI LEHEEQDEEE 1260
DEVPDDETVN QMIARHEEEF DLFMRMDLDR RREEARNPKR KPRLMEEDEL PSWIIKDDAE 1320
VERLTCEEEE EKMFGRGSRH RKEVDYSDSL TEKQWLKAIE EGTLEEIEEE VRQKKSSRKR 1380
KRDSEAGSST PTTSTRSRDK DEESKKQKKR GRPPAEKLSP NPPNLTKKMK KIVDAVIKYK 1440
DSSGRQLSEV FIQLPSRKEL PEYYELIRKP VDFKKIKERI RNHKYRSLND LEKDVMLLCQ 1500
NAQTFNLEGS LIYEDSIVLQ SVFTSVRQKI EKEDDSEGEE SEEEEEGEEE GSESESRSVK 1560
VKIKLGRKEK AQDRLKGGRR RPSRGSRAKP VVSDDDSEEE QEEDRSGSGS EED 1613 
Gene Ontology
 GO:0000792; C:heterochromatin; IDA:MGI.
 GO:0071565; C:nBAF complex; IDA:UniProtKB.
 GO:0071564; C:npBAF complex; IDA:UniProtKB.
 GO:0005719; C:nuclear euchromatin; IDA:MGI.
 GO:0005726; C:perichromatin fibrils; IDA:MGI.
 GO:0016514; C:SWI/SNF complex; IDA:MGI.
 GO:0071778; C:WINAC complex; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; IMP:MGI.
 GO:0003682; F:chromatin binding; IDA:MGI.
 GO:0008094; F:DNA-dependent ATPase activity; IEA:Compara.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
 GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IDA:MGI.
 GO:0001105; F:RNA polymerase II transcription coactivator activity; IEA:Compara.
 GO:0003714; F:transcription corepressor activity; ISS:UniProtKB.
 GO:0035887; P:aortic smooth muscle cell differentiation; IMP:MGI.
 GO:0001832; P:blastocyst growth; IMP:MGI.
 GO:0001835; P:blastocyst hatching; IMP:MGI.
 GO:0000902; P:cell morphogenesis; IMP:MGI.
 GO:0006338; P:chromatin remodeling; IMP:MGI.
 GO:0060318; P:definitive erythrocyte differentiation; IMP:MGI.
 GO:0010424; P:DNA methylation on cytosine within a CG sequence; IMP:MGI.
 GO:0035116; P:embryonic hindlimb morphogenesis; IMP:MGI.
 GO:0048562; P:embryonic organ morphogenesis; IMP:MGI.
 GO:0048730; P:epidermis morphogenesis; IMP:MGI.
 GO:0030198; P:extracellular matrix organization; IMP:MGI.
 GO:0030900; P:forebrain development; IMP:MGI.
 GO:0007403; P:glial cell fate determination; IMP:MGI.
 GO:0060347; P:heart trabecula formation; IGI:MGI.
 GO:0030902; P:hindbrain development; IMP:MGI.
 GO:0043966; P:histone H3 acetylation; IMP:MGI.
 GO:0030216; P:keratinocyte differentiation; IMP:MGI.
 GO:0001889; P:liver development; IMP:MGI.
 GO:0006346; P:methylation-dependent chromatin silencing; IDA:MGI.
 GO:0060766; P:negative regulation of androgen receptor signaling pathway; IEA:Compara.
 GO:0030308; P:negative regulation of cell growth; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0003407; P:neural retina development; IEA:Compara.
 GO:0006334; P:nucleosome assembly; TAS:MGI.
 GO:0006337; P:nucleosome disassembly; IEA:Compara.
 GO:0043923; P:positive regulation by host of viral transcription; IEA:Compara.
 GO:0043388; P:positive regulation of DNA binding; IGI:MGI.
 GO:0051091; P:positive regulation of sequence-specific DNA binding transcription factor activity; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:MGI.
 GO:0019827; P:stem cell maintenance; IMP:MGI.
 GO:0001570; P:vasculogenesis; IMP:MGI. 
Interpro
 IPR006576; BRK_domain.
 IPR001487; Bromodomain.
 IPR018359; Bromodomain_CS.
 IPR014978; Gln-Leu-Gln_QLQ.
 IPR013999; HAS_subgr.
 IPR014012; Helicase/SANT-assoc_DNA-bd.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR006562; HSA.
 IPR027417; P-loop_NTPase.
 IPR000330; SNF2_N. 
Pfam
 PF07533; BRK
 PF00439; Bromodomain
 PF00271; Helicase_C
 PF07529; HSA
 PF08880; QLQ
 PF00176; SNF2_N 
SMART
 SM00592; BRK
 SM00297; BROMO
 SM00487; DEXDc
 SM00490; HELICc
 SM00573; HSA
 SM00951; QLQ 
PROSITE
 PS00633; BROMODOMAIN_1
 PS50014; BROMODOMAIN_2
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51204; HSA
 PS51666; QLQ 
PRINTS
 PR00503; BROMODOMAIN.