CPLM-000384

Genbank Nucleotide ID

Importin subunit alpha-6

Protein Synonyms/Alias

Karyopherin subunit alpha-5

Homo sapiens (Human)

Position	Peptide	Type	References
110	KFRKLLSKEPNPPID	methylation	[1]
110	KFRKLLSKEPNPPID	ubiquitination	[2]
122	PIDQVIQKPGVVQRF	ubiquitination	[2]
131	GVVQRFVKFLERNEN	ubiquitination	[3, 4]
309	LLMHNDYKVVSPALR	ubiquitination	[2]
396	KAEFRTRKEAAWAIT	ubiquitination	[2, 4]

[1] Large-scale global identification of protein lysine methylation in vivo.
Cao XJ, Arnaudo AM, Garcia BA.
Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]

Functional Description

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates nuclear import of STAT1 homodimers and STAT1/STAT2 heterodimers by recognizing non- classical NLSs of STAT1 and STAT2 through ARM repeats 8-9. Recognizes influenza A virus nucleoprotein through ARM repeat 7-9 In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.

DOMAIN 2 57 IBB.
REPEAT 73 115 ARM 1; truncated.
REPEAT 116 159 ARM 2.
REPEAT 160 204 ARM 3.
REPEAT 205 243 ARM 4.
REPEAT 244 288 ARM 5.
REPEAT 289 328 ARM 6.
REPEAT 329 370 ARM 7.
REPEAT 371 410 ARM 8.
REPEAT 411 453 ARM 9.
REPEAT 457 502 ARM 10; atypical.
REGION 147 239 NLS binding site (major) (By similarity).
REGION 316 404 NLS binding site (minor) (By similarity).
MOTIF 42 51 Nuclear localization signal (By

Complete proteome; Cytoplasm; Polymorphism; Protein transport; Reference proteome; Repeat; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0005654; C:nucleoplasm; TAS:Reactome.
GO:0008565; F:protein transporter activity; IEA:InterPro.
GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
GO:0006607; P:NLS-bearing substrate import into nucleus; TAS:ProtInc.

IPR011989; ARM-like.
IPR016024; ARM-type_fold.
IPR000225; Armadillo.
IPR002652; Importin-a_IBB.
IPR024931; Importing_su_alpha.

PF00514; Arm
PF01749; IBB

PS50176; ARM_REPEAT
PS51214; IBB