CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011209
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vacuolar basic amino acid transporter 4 
Protein Synonyms/Alias
  
Gene Name
 VBA4 
Gene Synonyms/Alias
 YDR119W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
32RKSVQTLKNEVQREAubiquitination[1]
54QIALGNDKIEEINENubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, VillĂ©n J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301
Functional Description
 Transporter required for vacuolar uptake of basic amino acids. 
Sequence Annotation
 MOD_RES 62 62 Phosphoserine.
 MOD_RES 99 99 Phosphoserine.
 MOD_RES 106 106 Phosphoserine.
 MOD_RES 160 160 Phosphoserine.
 MOD_RES 192 192 Phosphoserine.
 CARBOHYD 480 480 N-linked (GlcNAc...) (Potential).
 CARBOHYD 553 553 N-linked (GlcNAc...) (Potential).  
Keyword
 Amino-acid transport; Coiled coil; Complete proteome; Glycoprotein; Membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport; Vacuole. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 768 AA 
Protein Sequence
MGKKDRQRKK LREFAKLKNR QRNLRKSVQT LKNEVQREAK VPRTSNQIAL GNDKIEEINE 60
NSPLLSAPSK QEEVSIPKAV DIDTIDAQPL HEGPKIDDSP QDEVNSIKGK PADKANEDDL 120
KPPSQHEACG NSALQSSITD FSDRSVSPLQ SITSCNTPMS EHELPVSSSN SFERADDMPV 180
VQADNQTSSS KSLHIVAPSP EVPVSGDEIT SYGYGSIPQS IGDVENGLNP PYVENTSSDE 240
LVHDLTRRRI FSSCMCTYLF FIAMDSSIIL VIASKIASEF HELWRLSLVI SAYLLSNAIG 300
QLVFLKLSLI SSVKLLLCIA QFSFILGGYL SWSSAHFWTF IFARCVTGFG GGSLIALKST 360
IMNRFSQKND SRYSLSASMI TFAMGVVIGP FMMNLFDSSH GSGWRNAFLI PVPFCLVNAS 420
IMLADMYSVK STLYGRPTPT LWKRFKNTLL SPDLYEILTL TLFLLCFVQV TSLDLTGLKN 480
NTMIQALLFS VIIVCGILFF LIETSDTYMN SVISMSLQGD KRLIWTMIGI SFCFAALMCI 540
IPFGTTYFII VLNLSTLQLA ERLSPFFFSI VLGYFSVSYF WKSKGQNFLL KFVLSGATLL 600
LYVALMGVSL NLPVWKQYIC LSLPFLGSSM ILTLLSNLYH EYHEQRKSPI SGSIVYCFGA 660
VGGTVGISLG GYVFHKTLIK LMHEKVMPFS KQGYLKKDLL KIIKHATESS DWVHESAPKF 720
VFQTLIECYL QACRNVFKLS TLFFTITVVA IFIFNRIHCR SQNCLSLS 768 
Gene Ontology
 GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
 GO:0055085; P:transmembrane transport; IEA:InterPro. 
Interpro
 IPR011701; MFS.
 IPR020846; MFS_dom.
 IPR016196; MFS_dom_general_subst_transpt. 
Pfam
 PF07690; MFS_1 
SMART
  
PROSITE
 PS50850; MFS 
PRINTS