CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018518
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CDGSH iron-sulfur domain-containing protein 1 
Protein Synonyms/Alias
 MitoNEET 
Gene Name
 Cisd1 
Gene Synonyms/Alias
 D10Ertd214e; Zcd1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
37AYKKFYAKENRTKAMacetylation[1]
55QIQKDNPKVVHAFDMacetylation[1, 2, 3, 4, 5]
55QIQKDNPKVVHAFDMubiquitination[6]
68DMEDLGDKAVYCRCWacetylation[1, 4, 5]
68DMEDLGDKAVYCRCWubiquitination[6]
104NVGPLIIKKKET***acetylation[1, 4, 5, 7, 8, 9]
104NVGPLIIKKKET***ubiquitination[6]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [6] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [7] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [8] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [9] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199
Functional Description
 Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation. May be involved in Fe-S cluster shuttling and/or in redox reactions (By similarity). 
Sequence Annotation
 METAL 72 72 Iron-sulfur (2Fe-2S) (By similarity).
 METAL 74 74 Iron-sulfur (2Fe-2S) (By similarity).
 METAL 83 83 Iron-sulfur (2Fe-2S) (By similarity).
 METAL 87 87 Iron-sulfur (2Fe-2S); via pros nitrogen
 MOD_RES 104 104 N6-acetyllysine.  
Keyword
 2Fe-2S; Acetylation; Complete proteome; Direct protein sequencing; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion outer membrane; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 108 AA 
Protein Sequence
MGLSSNSAVR VEWIAAVTFA AGTAALGYLA YKKFYAKENR TKAMVNLQIQ KDNPKVVHAF 60
DMEDLGDKAV YCRCWRSKKF PFCDGAHIKH NEETGDNVGP LIIKKKET 108 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005741; C:mitochondrial outer membrane; IDA:MGI.
 GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0005506; F:iron ion binding; ISO:MGI.
 GO:0043457; P:regulation of cellular respiration; IMP:MGI. 
Interpro
 IPR018967; FeS-contain_CDGSH-typ.
 IPR006622; FeS-contain_CDGSH-typ_subfam.
 IPR019610; FeS-contain_mitoNEET_N. 
Pfam
 PF10660; MitoNEET_N
 PF09360; zf-CDGSH 
SMART
 SM00704; ZnF_CDGSH 
PROSITE
  
PRINTS