CPLM-001464

Genbank Nucleotide ID

Actin-like protein 6B

Protein Synonyms/Alias

53 kDa BRG1-associated factor B; Actin-related protein Baf53b; ArpNalpha; BRG1-associated factor 53B; BAF53B

Homo sapiens (Human)

Position	Peptide	Type	References
368	LNRELSQKTPPSMRL	ubiquitination	[1, 2, 3, 4]
418	QEYEEGGKQCVERKC	ubiquitination	[2, 3, 4]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Belongs to the chromatin remodeling brain-specific BAF (bBAF) complex, as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex) and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a post- mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth (By similarity).

REGION 39 82 Essential for mediating its function in

Chromatin regulator; Complete proteome; Direct protein sequencing; Neurogenesis; Nucleus; Reference proteome; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0071565; C:nBAF complex; ISS:UniProtKB.
GO:0005730; C:nucleolus; IDA:HPA.
GO:0016514; C:SWI/SNF complex; ISS:UniProtKB.
GO:0005524; F:ATP binding; IEA:InterPro.
GO:0005200; F:structural constituent of cytoskeleton; TAS:ProtInc.
GO:0003713; F:transcription coactivator activity; NAS:BHF-UCL.
GO:0006338; P:chromatin remodeling; IDA:BHF-UCL.
GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
GO:0006357; P:regulation of transcription from RNA polymerase II promoter; NAS:BHF-UCL.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

IPR004000; Actin-related.
IPR004001; Actin_CS.