CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004033
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 
Protein Synonyms/Alias
 HMG-CoA reductase 1 
Gene Name
 HMG1 
Gene Synonyms/Alias
 YML075C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
619NTKQLKNKEVAALVIacetylation[1]
637LPLYALEKKLGDTTRacetylation[1]
726ASAMRGCKAINAGGGubiquitination[2]
740GATTVLTKDGMTRGPacetylation[1]
817MGMNMISKGVEYSLKacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 One of 2 isozymes that catalyze the conversion of HMG- CoA to mevalonate. It is the rate-limiting enzyme of the sterol biosynthesis pathway. Involved in ergosterol biosynthesis. 
Sequence Annotation
 DOMAIN 189 357 SSD.
 REGION 525 617 Linker.
 REGION 618 1054 Catalytic.
 ACT_SITE 714 714 Charge relay system (By similarity).
 ACT_SITE 848 848 Charge relay system (By similarity).
 ACT_SITE 924 924 Charge relay system (By similarity).
 ACT_SITE 1020 1020 Proton donor (By similarity).
 MOD_RES 552 552 Phosphothreonine.
 CARBOHYD 115 115 N-linked (GlcNAc...) (Potential).
 CARBOHYD 181 181 N-linked (GlcNAc...) (Potential).
 CARBOHYD 452 452 N-linked (GlcNAc...) (Potential).
 CARBOHYD 490 490 N-linked (GlcNAc...) (Potential).  
Keyword
 Complete proteome; Endoplasmic reticulum; Glycoprotein; Lipid biosynthesis; Lipid metabolism; Membrane; NADP; Nucleus; Oxidoreductase; Phosphoprotein; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1054 AA 
Protein Sequence
MPPLFKGLKQ MAKPIAYVSR FSAKRPIHII LFSLIISAFA YLSVIQYYFN GWQLDSNSVF 60
ETAPNKDSNT LFQECSHYYR DSSLDGWVSI TAHEASELPA PHHYYLLNLN FNSPNETDSI 120
PELANTVFEK DNTKYILQED LSVSKEISST DGTKWRLRSD RKSLFDVKTL AYSLYDVFSE 180
NVTQADPFDV LIMVTAYLMM FYTIFGLFND MRKTGSNFWL SASTVVNSAS SLFLALYVTQ 240
CILGKEVSAL TLFEGLPFIV VVVGFKHKIK IAQYALEKFE RVGLSKRITT DEIVFESVSE 300
EGGRLIQDHL LCIFAFIGCS MYAHQLKTLT NFCILSAFIL IFELILTPTF YSAILALRLE 360
MNVIHRSTII KQTLEEDGVV PSTARIISKA EKKSVSSFLN LSVVVIIMKL SVILLFVFIN 420
FYNFGANWVN DAFNSLYFDK ERVSLPDFIT SNASENFKEQ AIVSVTPLLY YKPIKSYQRI 480
EDMVLLLLRN VSVAIRDRFV SKLVLSALVC SAVINVYLLN AARIHTSYTA DQLVKTEVTK 540
KSFTAPVQKA STPVLTNKTV ISGSKVKSLS SAQSSSSGPS SSSEEDDSRD IESLDKKIRP 600
LEELEALLSS GNTKQLKNKE VAALVIHGKL PLYALEKKLG DTTRAVAVRR KALSILAEAP 660
VLASDRLPYK NYDYDRVFGA CCENVIGYMP LPVGVIGPLV IDGTSYHIPM ATTEGCLVAS 720
AMRGCKAINA GGGATTVLTK DGMTRGPVVR FPTLKRSGAC KIWLDSEEGQ NAIKKAFNST 780
SRFARLQHIQ TCLAGDLLFM RFRTTTGDAM GMNMISKGVE YSLKQMVEEY GWEDMEVVSV 840
SGNYCTDKKP AAINWIEGRG KSVVAEATIP GDVVRKVLKS DVSALVELNI AKNLVGSAMA 900
GSVGGFNAHA ANLVTAVFLA LGQDPAQNVE SSNCITLMKE VDGDLRISVS MPSIEVGTIG 960
GGTVLEPQGA MLDLLGVRGP HATAPGTNAR QLARIVACAV LAGELSLCAA LAAGHLVQSH 1020
MTHNRKPAEP TKPNNLDATD INRLKDGSVT CIKS 1054 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005635; C:nuclear envelope; IDA:SGD.
 GO:0034399; C:nuclear periphery; IDA:SGD.
 GO:0004420; F:hydroxymethylglutaryl-CoA reductase (NADPH) activity; IDA:SGD.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0015936; P:coenzyme A metabolic process; IEA:InterPro.
 GO:0006696; P:ergosterol biosynthetic process; IMP:SGD.
 GO:0019287; P:isopentenyl diphosphate biosynthetic process, mevalonate pathway; IC:SGD. 
Interpro
 IPR025583; HMG-CoA_N_dom.
 IPR002202; HMG_CoA_Rdtase.
 IPR023074; HMG_CoA_Rdtase_cat.
 IPR023076; HMG_CoA_Rdtase_CS.
 IPR004554; HMG_CoA_Rdtase_eu_arc.
 IPR023282; HMG_CoA_Rdtase_N.
 IPR009023; HMG_CoA_Rdtase_NAD(P)-bd.
 IPR009029; HMG_CoA_Rdtase_sub-bd.
 IPR000731; SSD. 
Pfam
 PF00368; HMG-CoA_red
 PF13323; HPIH 
SMART
  
PROSITE
 PS00066; HMG_COA_REDUCTASE_1
 PS00318; HMG_COA_REDUCTASE_2
 PS01192; HMG_COA_REDUCTASE_3
 PS50065; HMG_COA_REDUCTASE_4
 PS50156; SSD 
PRINTS
 PR00071; HMGCOARDTASE.