CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005599
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 X-ray repair cross-complementing protein 5 
Protein Synonyms/Alias
 ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku autoantigen protein p86 homolog; Ku80; Nuclear factor IV 
Gene Name
 Xrcc5 
Gene Synonyms/Alias
 G22p2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
265SIKIVAYKSIVQEKFacetylation[1]
325SDIIPFSKVDEEQMKubiquitination[2]
544FPLTEVIKKKNQVTAubiquitination[2]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription (By similarity). 
Sequence Annotation
 DOMAIN 251 460 Ku.
 REGION 138 165 Leucine-zipper.
 MOTIF 720 728 EEXXXDL motif.
 MOD_RES 265 265 N6-acetyllysine (By similarity).
 MOD_RES 332 332 N6-acetyllysine (By similarity).
 MOD_RES 578 578 Phosphoserine; by PRKDC (By similarity).
 MOD_RES 580 580 Phosphoserine; by PRKDC (By similarity).
 MOD_RES 581 581 Phosphoserine; by PRKDC (By similarity).
 MOD_RES 666 666 N6-acetyllysine (By similarity).
 MOD_RES 716 716 Phosphothreonine; by PRKDC (By  
Keyword
 Acetylation; Activator; ATP-binding; Chromosome; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 732 AA 
Protein Sequence
MAWSGNKAAV VLCVDVGVAM GNSFPGEESP IEQAKKVMTM FVQRQVFSES KDEIALVLYG 60
TDGTDNALAG KDQYQNITVC RHLMLPDFDL LEDIGNKIQP SSQQADFLDA LIVCMDLIQR 120
ETIGKKFGKK HIEVFTDLSS PFSQDQLDVI ICNLKKSGIS LQFFLPFPID KNGEPGERGD 180
LDSGLDHLKP SFPQKGLTEQ QKEGIRMVTR VMLSLEGEDG LDEIYSFSES LRQLCVFKKI 240
ERRSMPWPCQ LTIGPNLSIK IVAYKSIVQE KFKKSWVVVD ARTLKKEDIQ KETVYCLNDD 300
DETEVSKEDT IQGYRYGSDI IPFSKVDEEQ MKYKSEGKCF SVLGFCKSSQ VHRRFFMGHQ 360
VLKVFAAKDD EAAAVALSSL VHALDELNMV AIVRYAYDKR SNPQVGVAFP YIKDAYECLV 420
YVQLPFMEDL RQYMFSSLKN NKKCTPTEAQ LSAIDDLIDS MSLVKKNEEE DIVEDLFPTS 480
KIPNPEFQRL YQCLLHRALH LQERLPPIQQ HILNMLDPPT EMKAKCESPL SKVKTLFPLT 540
EVIKKKNQVT AQDVFQDNHE EGPAAKKYKT EKEEDHISIS SLAEGNITKV GSVNPVENFR 600
FLVRQKIASF EEASLQLISH IEQFLDTNET LYFMKSMDCI KAFREEAIQF SEEQRFNSFL 660
EALREKVEIK QLNHFWEIVV QDGVTLITKD EGPGSSITAE EATKFLAPKD KAKEDTTGPE 720
EAGDVDDLLD MI 732 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0043564; C:Ku70:Ku80 complex; ISS:UniProtKB.
 GO:0070419; C:nonhomologous end joining complex; ISS:UniProtKB.
 GO:0000784; C:nuclear chromosome, telomeric region; IEA:Compara.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0051575; F:5'-deoxyribose-5-phosphate lyase activity; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004003; F:ATP-dependent DNA helicase activity; IEA:InterPro.
 GO:0003684; F:damaged DNA binding; IEA:InterPro.
 GO:0003691; F:double-stranded telomeric DNA binding; IEA:Compara.
 GO:0044212; F:transcription regulatory region DNA binding; IEA:Compara.
 GO:0008283; P:cell proliferation; IMP:MGI.
 GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
 GO:0006303; P:double-strand break repair via nonhomologous end joining; ISS:UniProtKB.
 GO:0060218; P:hematopoietic stem cell differentiation; IMP:MGI.
 GO:0043066; P:negative regulation of apoptotic process; IMP:MGI.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; ISS:UniProtKB.
 GO:0050769; P:positive regulation of neurogenesis; IMP:MGI.
 GO:0000723; P:telomere maintenance; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR006164; Ku70/Ku80_beta-barrel_dom.
 IPR024193; Ku80.
 IPR005160; Ku_C.
 IPR005161; Ku_N.
 IPR014893; Ku_PK_bind.
 IPR016194; SPOC_like_C_dom.
 IPR002035; VWF_A. 
Pfam
 PF02735; Ku
 PF03730; Ku_C
 PF03731; Ku_N
 PF08785; Ku_PK_bind 
SMART
 SM00559; Ku78
 SM00327; VWA 
PROSITE
  
PRINTS