CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015325
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD repeat-containing protein 59 
Protein Synonyms/Alias
  
Gene Name
 WDR59 
Gene Synonyms/Alias
 KIAA1923; FP977 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
91RVDLYKWKDGSGEVGubiquitination[1]
140WDIKDTRKPTVALSAubiquitination[1]
159SQVKWNKKNANCLATubiquitination[1]
226WDYRQPRKYLNILPCubiquitination[1, 2]
239PCQVPVWKARYTPFSubiquitination[1]
298RKQKEGSKDYQLVTWubiquitination[1]
381RNLLEERKSDQLGLPubiquitination[3]
466AKLLKILKDTALQKVubiquitination[1]
588TGLIAPMKIRTEAPGubiquitination[1]
609GSPTRSEKEQVSISSubiquitination[1]
646RQIKAAGKVIIQDIAubiquitination[1]
679DIQETCQKNAASALLubiquitination[1]
863TQQFDDFKKCYGEILubiquitination[1, 3, 4]
864QQFDDFKKCYGEILYubiquitination[1]
894SCPPDPHKGIEFGVYubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
 REPEAT 57 98 WD 1.
 REPEAT 103 143 WD 2.
 REPEAT 146 185 WD 3.
 REPEAT 189 229 WD 4.
 REPEAT 232 276 WD 5.
 REPEAT 278 318 WD 6.
 REPEAT 319 362 WD 7.
 DOMAIN 393 494 RWD.
 REPEAT 668 706 WD 8.
 MOD_RES 821 821 Phosphoserine.
 MOD_RES 822 822 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Phosphoprotein; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 974 AA 
Protein Sequence
MAARWSSENV VVEFRDSQAT AMSVDCLGQH AVLSGRRFLY IVNLDAPFEG HRKISRQSKW 60
DIGAVQWNPH DSFAHYFAAS SNQRVDLYKW KDGSGEVGTT LQGHTRVISD LDWAVFEPDL 120
LVTSSVDTYI YIWDIKDTRK PTVALSAVAG ASQVKWNKKN ANCLATSHDG DVRIWDKRKP 180
STAVEYLAAH LSKIHGLDWH PDSEHILATS SQDNSVKFWD YRQPRKYLNI LPCQVPVWKA 240
RYTPFSNGLV TVMVPQLRRE NSLLLWNVFD LNTPVHTFVG HDDVVLEFQW RKQKEGSKDY 300
QLVTWSRDQT LRMWRVDSQM QRLCANDILD GVDEFIESIS LLPEPEKTLH TEDTDHQHTA 360
SHGEEEALKE DPPRNLLEER KSDQLGLPQT LQQEFSLINV QIRNVNVEMD AADRSCTVSV 420
HCSNHRVKML VKFPAQYPNN AAPSFQFINP TTITSTMKAK LLKILKDTAL QKVKRGQSCL 480
EPCLRQLVSC LESFVNQEDS ASSNPFALPN SVTPPLPTFA RVTTAYGSYQ DANIPFPRTS 540
GARFCGAGYL VYFTRPMTMH RAVSPTEPTP RSLSALSAYH TGLIAPMKIR TEAPGNLRLY 600
SGSPTRSEKE QVSISSFYYK ERKSRRWKSK REGSDSGNRQ IKAAGKVIIQ DIACLLPVHK 660
SLGELYILNV NDIQETCQKN AASALLVGRK DLVQVWSLAT VATDLCLGPK SDPDLETPWA 720
RHPFGRQLLE SLLAHYCRLR DVQTLAMLCS VFEAQSRPQG LPNPFGPFPN RSSNLVVSHS 780
RYPSFTSSGS CSSMSDPGLN TGGWNIAGRE AEHLSSPWGE SSPEELRFGS LTYSDPRERE 840
RDQHDKNKRL LDPANTQQFD DFKKCYGEIL YRWGLREKRA EVLKFVSCPP DPHKGIEFGV 900
YCSHCRSEVR GTQCAICKGF TFQCAICHVA VRGSSNFCLT CGHGGHTSHM MEWFRTQEVC 960
PTGCGCHCLL ESTF 974 
Gene Ontology
  
Interpro
 IPR006575; RWD-domain.
 IPR016135; UBQ-conjugating_enzyme/RWD.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00591; RWD
 SM00320; WD40 
PROSITE
 PS50908; RWD
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS