CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023907
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin carboxyl-terminal hydrolase isozyme L5 
Protein Synonyms/Alias
 UCH-L5; Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5 
Gene Name
 UCHL5 
Gene Synonyms/Alias
 UCH37; AD-019; CGI-70 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
126QSFDAAMKGLALSNSubiquitination[1]
154QMFEFDTKTSAKEEDubiquitination[1, 2]
158FDTKTSAKEEDAFHFacetylation[3]
158FDTKTSAKEEDAFHFubiquitination[1, 2]
210VIEKRIQKYSEGEIRubiquitination[1]
234RKMIYEQKIAELQRQubiquitination[1, 2]
277LIEEEVQKLKRYKIEubiquitination[1]
315QLIPLVEKAKEKQNAubiquitination[1, 2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1. 
Sequence Annotation
 REGION 313 329 Interaction with ADRM1.
 ACT_SITE 88 88 Nucleophile (Probable).
 ACT_SITE 164 164 Proton donor (By similarity).
 MOD_RES 158 158 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; DNA damage; DNA recombination; DNA repair; Hydrolase; Nucleus; Polymorphism; Protease; Proteasome; Reference proteome; Thiol protease; Transcription; Transcription regulation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 329 AA 
Protein Sequence
MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP 60
GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS 120
FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG 180
LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR 240
QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL 300
KTLAEHQQLI PLVEKAKEKQ NAKKAQETK 329 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0031011; C:Ino80 complex; IDA:UniProtKB.
 GO:0000502; C:proteasome complex; IEA:UniProtKB-KW.
 GO:0004866; F:endopeptidase inhibitor activity; IMP:UniProtKB.
 GO:0008242; F:omega peptidase activity; IEA:InterPro.
 GO:0070628; F:proteasome binding; IDA:UniProtKB.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0004843; F:ubiquitin-specific protease activity; IDA:UniProtKB.
 GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0048853; P:forebrain morphogenesis; IEA:Compara.
 GO:0021670; P:lateral ventricle development; IEA:Compara.
 GO:0030901; P:midbrain development; IEA:Compara.
 GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; TAS:Reactome.
 GO:0016579; P:protein deubiquitination; IDA:UniProtKB.
 GO:0061136; P:regulation of proteasomal protein catabolic process; IMP:UniProtKB.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; TAS:Reactome.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR001578; Peptidase_C12.
 IPR017390; Ubiquitinyl_hydrolase_UCH37. 
Pfam
 PF01088; Peptidase_C12 
SMART
  
PROSITE
 PS00140; UCH_1 
PRINTS
 PR00707; UBCTHYDRLASE.