CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016509
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pyruvate dehydrogenase protein X component, mitochondrial 
Protein Synonyms/Alias
 Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; Lipoyl-containing pyruvate dehydrogenase complex component X 
Gene Name
 Pdhx 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
194AARNILEKHSLDASQacetylation[1]
213GPRGIFTKEDALKLVacetylation[1]
218FTKEDALKLVELKQMacetylation[1]
321KVRRDLVKDDIKVSVacetylation[1, 2]
325DLVKDDIKVSVNDFIacetylation[1, 2]
394QEIADSVKVLSKKARacetylation[1, 2, 3, 4]
394QEIADSVKVLSKKARsuccinylation[4]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex (By similarity). 
Sequence Annotation
 DOMAIN 57 131 Lipoyl-binding.
 REGION 183 214 Interaction with DLD (By similarity).
 MOD_RES 97 97 N6-lipoyllysine (By similarity).
 MOD_RES 194 194 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Complete proteome; Lipoyl; Mitochondrion; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 501 AA 
Protein Sequence
MAASWRLHCN QPLLRYLLGF SSRRSLGLAQ GAAAWPVDRG ASWRWFHSTQ LLQADPIKVL 60
MPSLSPTMEQ GNIVKWLRKE GEAVSAGDSL CEIETDKAVV TLDANDDGIL AKIVVEEGAK 120
NIQLGSLIAL MVEEGEDWKQ VEIPKDVSAP PPVSKPPAPT QPSPQPQIPC PARKEHKGTA 180
RFRLSPAARN ILEKHSLDAS QGTATGPRGI FTKEDALKLV ELKQMGKITE SRPASAPPPS 240
LSASVPPQAT AGPSYPRPMT PPVSIPGQPN AAGTFTEIPA SNIRRVIAKR LTESKSTVPH 300
AYATADCDLG AVLKVRRDLV KDDIKVSVND FIIRAAAVTL KQMPGVNVTW DGEGPKQLPS 360
VDISVAVATD KGLITPIIKD AAAKGIQEIA DSVKVLSKKA RDGKLMPEEY QGGSFSISNL 420
GMFGIDEFAA VINPPQACIL AVGRFRPVLK LTEDEEGNPQ LQQHQLITVT MSSDSRVVDD 480
ELATRFLETF KANLENPMRL G 501 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0016746; F:transferase activity, transferring acyl groups; IEA:InterPro. 
Interpro
 IPR003016; 2-oxoA_DH_lipoyl-BS.
 IPR001078; 2-oxoacid_DH_actylTfrase.
 IPR000089; Biotin_lipoyl.
 IPR023213; CAT-like_dom.
 IPR004167; E3-bd.
 IPR011053; Single_hybrid_motif. 
Pfam
 PF00198; 2-oxoacid_dh
 PF00364; Biotin_lipoyl
 PF02817; E3_binding 
SMART
  
PROSITE
 PS50968; BIOTINYL_LIPOYL
 PS00189; LIPOYL 
PRINTS