CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008166
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable histidine--tRNA ligase, mitochondrial 
Protein Synonyms/Alias
 Histidine--tRNA ligase-like; Histidyl-tRNA synthetase; HisRS 
Gene Name
 HARS2 
Gene Synonyms/Alias
 HARSL; HARSR; HO3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
119SGLMYDLKDQGGELLubiquitination[1]
419VFVATPQKNFLQERLubiquitination[1]
444IKAEMLYKNNPKLLTacetylation[2]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
  
Sequence Annotation
 MOD_RES 67 67 Phosphoserine.
 MOD_RES 444 444 N6-acetyllysine.  
Keyword
 Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Deafness; Disease mutation; Ligase; Mitochondrion; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 506 AA 
Protein Sequence
MPLLGLLPRR AWASLLSQLL RPPCASCTGA VRCQSQVAEA VLTSQLKAHQ EKPNFIIKTP 60
KGTRDLSPQH MVVREKILDL VISCFKRHGA KGMDTPAFEL KETLTEKYGE DSGLMYDLKD 120
QGGELLSLRY DLTVPFARYL AMNKVKKMKR YHVGKVWRRE SPTIVQGRYR EFCQCDFDIA 180
GQFDPMIPDA ECLKIMCEIL SGLQLGDFLI KVNDRRIVDG MFAVCGVPES KFRAICSSID 240
KLDKMAWKDV RHEMVVKKGL APEVADRIGD YVQCHGGVSL VEQMFQDPRL SQNKQALEGL 300
GDLKLLFEYL TLFGIADKIS FDLSLARGLD YYTGVIYEAV LLQTPTQAGE EPLNVGSVAA 360
GGRYDGLVGM FDPKGHKVPC VGLSIGVERI FYIVEQRMKT KGEKVRTTET QVFVATPQKN 420
FLQERLKLIA ELWDSGIKAE MLYKNNPKLL TQLHYCESTG IPLVVIIGEQ ELKEGVIKIR 480
SVASREEVAI KRENFVAEIQ KRLSES 506 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0005524; F:ATP binding; NAS:UniProtKB.
 GO:0004821; F:histidine-tRNA ligase activity; NAS:UniProtKB.
 GO:0006427; P:histidyl-tRNA aminoacylation; IEA:InterPro.
 GO:0006418; P:tRNA aminoacylation for protein translation; TAS:Reactome. 
Interpro
 IPR002314; aa-tRNA-synt_IIb_cons-dom.
 IPR006195; aa-tRNA-synth_II.
 IPR004154; Anticodon-bd.
 IPR015807; His-tRNA-ligase.
 IPR004516; HisRS/HisZ. 
Pfam
 PF03129; HGTP_anticodon
 PF00587; tRNA-synt_2b 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS