CPLM-012446

Genbank Nucleotide ID

Signal peptidase complex subunit 2

Protein Synonyms/Alias

Microsomal signal peptidase 25 kDa subunit; SPase 25 kDa subunit

Homo sapiens (Human)

Position	Peptide	Type	References
38	GRSGLLDKWKIDDKP	ubiquitination	[1, 2]
57	KWDGSAVKNSLDDSA	ubiquitination	[2, 3]
66	SLDDSAKKVLLEKYK	ubiquitination	[3]
71	AKKVLLEKYKYVENF	ubiquitination	[2, 3]
146	IFLVAHRKDPTGMDP	ubiquitination	[2, 3]
164	WQLSSSLKRFDDKYT	ubiquitination	[2, 3, 4]
169	SLKRFDDKYTLKLTF	acetylation	[5]
169	SLKRFDDKYTLKLTF	ubiquitination	[2]
191	QREAEFTKSIAKFFD	acetylation	[5]
191	QREAEFTKSIAKFFD	ubiquitination	[2, 3, 4, 6]
195	EFTKSIAKFFDHSGT	ubiquitination	[2, 7, 8]

[1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum (By similarity).

MOD_RES 2 2 N-acetylalanine.
MOD_RES 169 169 N6-acetyllysine.
MOD_RES 191 191 N6-acetyllysine.

Acetylation; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Hydrolase; Membrane; Microsome; Protease; Reference proteome; Transmembrane; Transmembrane helix.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005787; C:signal peptidase complex; IEA:InterPro.
GO:0008233; F:peptidase activity; IEA:UniProtKB-KW.
GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
GO:0050796; P:regulation of insulin secretion; TAS:Reactome.
GO:0006465; P:signal peptide processing; IEA:InterPro.
GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
GO:0006412; P:translation; TAS:Reactome.

IPR009582; SigPept_cplx_su2.