UniProt Accession

 HDA_ECOLI; P69931; P76570; P76979 

Genbank Protein ID

 U00096; AP009048 

Genbank Nucleotide ID

 AAC75549.2; BAA16384.1 

Protein Name

 DnaA regulatory inactivator Hda 

Protein Synonyms/Alias

 DnaA paralog; Dp 

Gene Name

 hda 

Gene Synonyms/Alias

 idaB; yfgE; b2496; JW5397; f248c 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
161	LDWGQIYKLQPLSDE	acetylation	[1]
170	QPLSDEDKLQALQLR	acetylation	[1]
228	KLTIPFVKEILKL**	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Mediates the interactions of DNA replication inititator protein DnaA with DNA polymerase subunit beta sliding clamp (dnaN). Stimulates hydrolysis of ATP-DnaA to ADP-DnaA, rendering DnaA inactive for reinititation, a process called regulatory inhibition of DnaA or RIDA. ADP-binding activates Hda to hydrolyze DnaA-ATP; Hda monomers bind to ADP with about 200-fold greater affinity than for ATP. RIDA function can be genetically separated from viability, suggesting this protein has another function as well. 

Sequence Annotation

 REGION 6 11 Beta clamp binding motif.  

Keyword

 Cell inner membrane; Cell membrane; Complete proteome; Direct protein sequencing; DNA replication; DNA replication inhibitor; Membrane; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 233 AA 

Protein Sequence

Gene Ontology

 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
 GO:0006260; P:DNA replication; IEA:HAMAP.
 GO:0032297; P:negative regulation of DNA-dependent DNA replication initiation; IMP:EcoCyc. 

Interpro

 IPR020591; Chromosome_initiator_DnaA-like.
 IPR017788; Chromosome_reg_Hda.
 IPR013317; DnaA_Hda.
 IPR022864; Hda_Enterobacteriaceae.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00308; Bac_DnaA 

SMART

  

PROSITE

  

PRINTS

 PR00051; DNAA.