UniProt Accession

 NSUN5_HUMAN; Q96P11; B3KX04; B4DP79; G3V0G9; Q6ZUI8; Q96HT9; Q9NW70 

Genbank Protein ID

 AF420249; AF412028; AK001129; AK125667; AK126375; AK298221; AC073841; AC073841; CH471200; BC008084 

Genbank Nucleotide ID

 AAL16067.1; AAM62310.1; BAA91515.1; BAG54316.1; BAG60491.1; AAQ96838.1; AAQ96839.1; EAW69694.1; AAH08084.1 

Protein Name

 Putative methyltransferase NSUN5 

Protein Synonyms/Alias

 NOL1-related protein; NOL1R; NOL1/NOP2/Sun domain family member 5; Williams-Beuren syndrome chromosomal region 20A protein 

Gene Name

 NSUN5 

Gene Synonyms/Alias

 WBSCR20; WBSCR20A 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
22	ESRQGSIKGLVYSSN	ubiquitination	[1]
82	VYELLLGKGFRGGGG	ubiquitination	[1, 2]
92	RGGGGRWKALLGRHQ	ubiquitination	[1]
103	GRHQARLKAELARLK	ubiquitination	[1]
144	FVRVNTLKTCSDDVV	ubiquitination	[1]
155	DDVVDYFKRQGFSYQ	ubiquitination	[1]
177	DLRALKGKHFLLDPL	ubiquitination	[1]
249	SHLAALLKNQGKIFA	ubiquitination	[1]
253	ALLKNQGKIFAFDLD	ubiquitination	[1, 2]
262	FAFDLDAKRLASMAT	ubiquitination	[1, 2]
435	PSSASQAKASAPERT	ubiquitination	[1, 2, 3, 4, 5]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 May have S-adenosyl-L-methionine-dependent methyl- transferase activity (Potential). 

Sequence Annotation

 REGION 234 240 S-adenosyl-L-methionine binding.
 REGION 285 286 S-adenosyl-L-methionine binding.
 ACT_SITE 359 359 Nucleophile (Potential).
 BINDING 258 258 S-adenosyl-L-methionine.
 BINDING 263 263 S-adenosyl-L-methionine.
 BINDING 305 305 S-adenosyl-L-methionine.
 MOD_RES 2 2 N-acetylglycine.
 MOD_RES 167 167 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Methyltransferase; Phosphoprotein; Polymorphism; Reference proteome; S-adenosyl-L-methionine; Transferase; Williams-Beuren syndrome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 429 AA 

Protein Sequence

Gene Ontology

 GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW. 

Interpro

 IPR001678; Fmu/NOL1/Nop2p.
 IPR023267; RCMT. 

Pfam

 PF01189; Nol1_Nop2_Fmu 

SMART

  

PROSITE

 PS01153; NOL1_NOP2_SUN 

PRINTS

 PR02008; RCMTFAMILY.