CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022282
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glucose-induced degradation protein 8 homolog 
Protein Synonyms/Alias
 Two hybrid-associated protein 1 with RanBPM; Twa1 
Gene Name
 GID8 
Gene Synonyms/Alias
 C20orf11 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
6**MSYAEKPDEITKDubiquitination[1]
171LHTMQRQKVWSEVNQubiquitination[1, 2, 3, 4]
197PKLAKLLKLLLWAQNubiquitination[1, 3]
221PKMTDLSKGVIEEPKubiquitination[3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
 DOMAIN 25 57 LisH.
 DOMAIN 63 120 CTLH.  
Keyword
 Coiled coil; Complete proteome; Nucleus; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 228 AA 
Protein Sequence
MSYAEKPDEI TKDEWMEKLN NLHVQRADMN RLIMNYLVTE GFKEAAEKFR MESGIEPSVD 60
LETLDERIKI REMILKGQIQ EAIALINSLH PELLDTNRYL YFHLQQQHLI ELIRQRETEA 120
ALEFAQTQLA EQGEESRECL TEMERTLALL AFDSPEESPF GDLLHTMQRQ KVWSEVNQAV 180
LDYENRESTP KLAKLLKLLL WAQNELDQKK VKYPKMTDLS KGVIEEPK 228 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. 
Interpro
 IPR013144; CRA_dom.
 IPR024964; CTLH/CRA.
 IPR006595; CTLH_C.
 IPR006594; LisH_dimerisation.
 IPR013720; LisH_dimerisation_subgr. 
Pfam
 PF10607; CLTH
 PF08513; LisH 
SMART
 SM00757; CRA
 SM00668; CTLH
 SM00667; LisH 
PROSITE
 PS50897; CTLH
 PS50896; LISH 
PRINTS