CPLM-002366

Genbank Nucleotide ID

Protein Synonyms/Alias

2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein

Homo sapiens (Human)

Position	Peptide	Type	References
10	EMDGTENKSKFGANA	ubiquitination	[1]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

REGION 31 38 Epitope recognized by CAR and healthy
REGION 56 63 Epitope recognized by CAR antibodies.
REGION 97 237 Required for repression of c-myc promoter
REGION 370 373 Substrate binding (By similarity).
REGION 405 434 Required for interaction with PLG (By
ACT_SITE 210 210 Proton donor (By similarity).
ACT_SITE 343 343 Proton acceptor (By similarity).
METAL 245 245 Magnesium (By similarity).
METAL 293 293 Magnesium (By similarity).
METAL 318 318 Magnesium (By similarity).
BINDING 158 158 Substrate (By similarity).
BINDING 167 167 Substrate (By similarity).
BINDING 293 293 Substrate (By similarity).
BINDING 318 318 Substrate (By similarity).
BINDING 394 394 Substrate (By similarity).
MOD_RES 2 2 N-acetylserine.
MOD_RES 5 5 N6-acetyllysine.
MOD_RES 44 44 Phosphotyrosine.
MOD_RES 64 64 N6-acetyllysine.
MOD_RES 71 71 N6-acetyllysine.
MOD_RES 89 89 N6-acetyllysine.
MOD_RES 126 126 N6-acetyllysine.
MOD_RES 193 193 N6-acetyllysine.
MOD_RES 199 199 N6-acetyllysine.
MOD_RES 228 228 N6-acetyllysine.
MOD_RES 233 233 N6-acetyllysine; alternate.
MOD_RES 233 233 N6-malonyllysine; alternate.
MOD_RES 254 254 Phosphoserine.
MOD_RES 256 256 N6-acetyllysine.
MOD_RES 263 263 Phosphoserine.
MOD_RES 272 272 Phosphoserine.
MOD_RES 281 281 N6-acetyllysine.
MOD_RES 285 285 N6-acetyllysine.
MOD_RES 287 287 Phosphotyrosine.
MOD_RES 420 420 N6-acetyllysine; alternate.
MOD_RES 420 420 N6-malonyllysine; alternate.

3D-structure; Acetylation; Alternative initiation; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Glycolysis; Lyase; Magnesium; Membrane; Metal-binding; Nucleus; Phosphoprotein; Plasminogen activation; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0070062; C:extracellular vesicular exosome; IDA:UniProtKB.
GO:0031430; C:M band; IEA:UniProtKB-SubCell.
GO:0005634; C:nucleus; IC:UniProtKB.
GO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro.
GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
GO:0000287; F:magnesium ion binding; IEA:InterPro.
GO:0004634; F:phosphopyruvate hydratase activity; TAS:ProtInc.
GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
GO:0006094; P:gluconeogenesis; TAS:Reactome.
GO:0006096; P:glycolysis; TAS:Reactome.
GO:0030308; P:negative regulation of cell growth; IDA:UniProtKB.
GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; TAS:ProtInc.
GO:0009615; P:response to virus; IEP:UniProtKB.
GO:0044281; P:small molecule metabolic process; TAS:Reactome.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

IPR000941; Enolase.
IPR020810; Enolase_C.
IPR020809; Enolase_CS.
IPR020811; Enolase_N.

PF00113; Enolase_C
PF03952; Enolase_N