UniProt Accession

 DYR_RAT; Q920D2 

Genbank Protein ID

 AF318150 

Genbank Nucleotide ID

 AAL11500.1 

Protein Name

 Dihydrofolate reductase 

Protein Synonyms/Alias

  

Gene Name

 Dhfr 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
33	PLLRNEFKYFQRMTT	acetylation	[1]
99	KSLDDALKLIEQPEL	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1 (By similarity). 

Sequence Annotation

 DOMAIN 4 185 DHFR.
 NP_BIND 16 22 NADP (By similarity).
 NP_BIND 55 57 NADP (By similarity).
 NP_BIND 77 79 NADP (By similarity).
 NP_BIND 117 124 NADP (By similarity).
 REGION 31 36 Substrate binding (By similarity).
 BINDING 10 10 NADP; via amide nitrogen and carbonyl
 BINDING 71 71 Substrate (By similarity).  

Keyword

 Complete proteome; Methotrexate resistance; NADP; One-carbon metabolism; Oxidoreductase; Reference proteome; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 187 AA 

Protein Sequence

Gene Ontology

 GO:0004146; F:dihydrofolate reductase activity; ISS:UniProtKB.
 GO:0051871; F:dihydrofolic acid binding; IDA:RGD.
 GO:0008144; F:drug binding; ISS:UniProtKB.
 GO:0003729; F:mRNA binding; ISS:UniProtKB.
 GO:0050661; F:NADP binding; IDA:RGD.
 GO:0046452; P:dihydrofolate metabolic process; IDA:RGD.
 GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
 GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro.
 GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
 GO:0031427; P:response to methotrexate; IEA:UniProtKB-KW.
 GO:0035094; P:response to nicotine; IEP:RGD.
 GO:0046654; P:tetrahydrofolate biosynthetic process; IDA:RGD. 

Interpro

 IPR012259; DHFR.
 IPR024072; DHFR-like_dom.
 IPR017925; DHFR_CS.
 IPR001796; DHFR_dom. 

Pfam

 PF00186; DHFR_1 

SMART

  

PROSITE

 PS00075; DHFR_1
 PS51330; DHFR_2 

PRINTS

 PR00070; DHFR.