CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021333
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable serine carboxypeptidase CPVL 
Protein Synonyms/Alias
 Carboxypeptidase, vitellogenic-like; Vitellogenic carboxypeptidase-like protein; VCP-like protein; hVLP 
Gene Name
 CPVL 
Gene Synonyms/Alias
 VLP; PSEC0124; UNQ197/PRO223 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
269KQKKYFQKQCHECIEubiquitination[1]
280ECIEHIRKQNWFEAFubiquitination[1, 2, 3]
331EDQLYYVKFLSLPEVubiquitination[1, 2, 3]
406SLMGMDWKGSQEYKKubiquitination[1]
423KKVWKIFKSDSEVAGubiquitination[2, 3]
469INRFIYGKGWDPYVGubiquitination[1, 4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 May be involved in the digestion of phagocytosed particles in the lysosome, participation in an inflammatory protease cascade, and trimming of peptides for antigen presentation. 
Sequence Annotation
 ACT_SITE 204 204 By similarity.
 ACT_SITE 388 388 By similarity.
 ACT_SITE 448 448 By similarity.
 CARBOHYD 81 81 N-linked (GlcNAc...).
 CARBOHYD 132 132 N-linked (GlcNAc...).
 CARBOHYD 307 307 N-linked (GlcNAc...) (Potential).
 CARBOHYD 346 346 N-linked (GlcNAc...).  
Keyword
 Carboxypeptidase; Complete proteome; Glycoprotein; Hydrolase; Polymorphism; Protease; Reference proteome; Signal; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 476 AA 
Protein Sequence
MVGAMWKVIV SLVLLMPGPC DGLFRSLYRS VSMPPKGDSG QPLFLTPYIE AGKIQKGREL 60
SLVGPFPGLN MKSYAGFLTV NKTYNSNLFF WFFPAQIQPE DAPVVLWLQG GPGGSSMFGL 120
FVEHGPYVVT SNMTLRDRDF PWTTTLSMLY IDNPVGTGFS FTDDTHGYAV NEDDVARDLY 180
SALIQFFQIF PEYKNNDFYV TGESYAGKYV PAIAHLIHSL NPVREVKINL NGIAIGDGYS 240
DPESIIGGYA EFLYQIGLLD EKQKKYFQKQ CHECIEHIRK QNWFEAFEIL DKLLDGDLTS 300
DPSYFQNVTG CSNYYNFLRC TEPEDQLYYV KFLSLPEVRQ AIHVGNQTFN DGTIVEKYLR 360
EDTVQSVKPW LTEIMNNYKV LIYNGQLDII VAAALTERSL MGMDWKGSQE YKKAEKKVWK 420
IFKSDSEVAG YIRQAGDFHQ VIIRGGGHIL PYDQPLRAFD MINRFIYGKG WDPYVG 476 
Gene Ontology
 GO:0004185; F:serine-type carboxypeptidase activity; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR001563; Peptidase_S10.
 IPR018202; Peptidase_S10_AS. 
Pfam
 PF00450; Peptidase_S10 
SMART
  
PROSITE
 PS00560; CARBOXYPEPT_SER_HIS
 PS00131; CARBOXYPEPT_SER_SER 
PRINTS
 PR00724; CRBOXYPTASEC.