CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044220
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Eukaryotic translation initiation factor 5A-1 
Protein Synonyms/Alias
  
Gene Name
 EIF5A 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
121LPEGDLGKEIEQKYDacetylation[1, 2]
121LPEGDLGKEIEQKYDubiquitination[2, 3, 4, 5, 6, 7, 8]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [8] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 147 AA 
Protein Sequence
MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG 60
IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS LLQDSGEVRE DLRLPEGDLG 120
KEIEQKYDCG EEILITVLSA MTEEAAV 147 
Gene Ontology
 GO:0043022; F:ribosome binding; IEA:InterPro.
 GO:0003746; F:translation elongation factor activity; IEA:InterPro.
 GO:0008612; P:peptidyl-lysine modification to hypusine; IEA:InterPro.
 GO:0045901; P:positive regulation of translational elongation; IEA:InterPro.
 GO:0045905; P:positive regulation of translational termination; IEA:InterPro.
 GO:0006452; P:translational frameshifting; IEA:InterPro. 
Interpro
 IPR005824; KOW.
 IPR012340; NA-bd_OB-fold.
 IPR014722; Rib_L2_dom2.
 IPR019769; Trans_elong_IF5A_hypusine_site.
 IPR001884; Transl_elong_IF5A.
 IPR020189; Transl_elong_IF5A_C.
 IPR008991; Translation_prot_SH3-like. 
Pfam
 PF01287; eIF-5a
 PF00467; KOW 
SMART
  
PROSITE
 PS00302; IF5A_HYPUSINE 
PRINTS