CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003720
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Heat shock protein SSC1, mitochondrial 
Protein Synonyms/Alias
 Endonuclease SceI 75 kDa subunit; Endo.SceI 75 kDa subunit; mtHSP70 
Gene Name
 SSC1 
Gene Synonyms/Alias
 ENS1; YJR045C; J1639 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
5***MLAAKNILNRSSacetylation[1]
53IMEGKVPKIIENAEGacetylation[1]
72PSVVAFTKEGERLVGacetylation[1]
98ENTLFATKRLIGRRFacetylation[1]
115AEVQRDIKQVPYKIVacetylation[1]
120DIKQVPYKIVKHSNGacetylation[1]
123QVPYKIVKHSNGDAWacetylation[1]
161TAEAYLGKPVKNAVVacetylation[1]
183DSQRQATKDAGQIVGacetylation[1]
211ALAYGLEKSDSKVVAacetylation[1]
268REIVSRFKTETGIDLacetylation[1]
322GPKHINMKFSRAQFEacetylation[1]
337TLTAPLVKRTVDPVKacetylation[1]
348DPVKKALKDAGLSTSacetylation[1]
371GGMSRMPKVVETVKSacetylation[1]
377PKVVETVKSLFGKDPacetylation[1]
382TVKSLFGKDPSKAVNacetylation[1]
561QAIETANKADQLANDacetylation[1]
574NDTENSLKEFEGKVDacetylation[1]
579SLKEFEGKVDKAEAQacetylation[1]
596RDQITSLKELVARVQacetylation[1]
614EVNAEELKTKTEELQacetylation[1]
626ELQTSSMKLFEQLYKacetylation[1]
633KLFEQLYKNDSNNNNacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Constitutes the ATP-driven core of the motor and binds the precursor preprotein. Required for the import of the processed fratxin homolog YFH1 into the mitochondrion. 
Sequence Annotation
 MOD_RES 330 330 Phosphothreonine.  
Keyword
 ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Stress response; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 654 AA 
Protein Sequence
MLAAKNILNR SSLSSSFRIA TRLQSTKVQG SVIGIDLGTT NSAVAIMEGK VPKIIENAEG 60
SRTTPSVVAF TKEGERLVGI PAKRQAVVNP ENTLFATKRL IGRRFEDAEV QRDIKQVPYK 120
IVKHSNGDAW VEARGQTYSP AQIGGFVLNK MKETAEAYLG KPVKNAVVTV PAYFNDSQRQ 180
ATKDAGQIVG LNVLRVVNEP TAAALAYGLE KSDSKVVAVF DLGGGTFDIS ILDIDNGVFE 240
VKSTNGDTHL GGEDFDIYLL REIVSRFKTE TGIDLENDRM AIQRIREAAE KAKIELSSTV 300
STEINLPFIT ADASGPKHIN MKFSRAQFET LTAPLVKRTV DPVKKALKDA GLSTSDISEV 360
LLVGGMSRMP KVVETVKSLF GKDPSKAVNP DEAVAIGAAV QGAVLSGEVT DVLLLDVTPL 420
SLGIETLGGV FTRLIPRNTT IPTKKSQIFS TAAAGQTSVE IRVFQGEREL VRDNKLIGNF 480
TLAGIPPAPK GVPQIEVTFD IDADGIINVS ARDKATNKDS SITVAGSSGL SENEIEQMVN 540
DAEKFKSQDE ARKQAIETAN KADQLANDTE NSLKEFEGKV DKAEAQKVRD QITSLKELVA 600
RVQGGEEVNA EELKTKTEEL QTSSMKLFEQ LYKNDSNNNN NNNGNNAESG ETKQ 654 
Gene Ontology
 GO:0042645; C:mitochondrial nucleoid; IDA:SGD.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0001405; C:presequence translocase-associated import motor; IDA:SGD.
 GO:0072380; C:TRC complex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; IDA:SGD.
 GO:0030234; F:enzyme regulator activity; IDA:SGD.
 GO:0032079; P:positive regulation of endodeoxyribonuclease activity; IDA:SGD.
 GO:0030150; P:protein import into mitochondrial matrix; IDA:SGD.
 GO:0042026; P:protein refolding; IDA:SGD.
 GO:0043335; P:protein unfolding; IMP:SGD.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW. 
Interpro
 IPR012725; Chaperone_DnaK.
 IPR018181; Heat_shock_70_CS.
 IPR013126; Hsp_70_fam. 
Pfam
 PF00012; HSP70 
SMART
  
PROSITE
 PS00297; HSP70_1
 PS00329; HSP70_2
 PS01036; HSP70_3 
PRINTS
 PR00301; HEATSHOCK70.