UniProt Accession

 UCHL1_MOUSE; Q9R0P9; Q9R122 

Genbank Protein ID

 AB025313; AF172334; AK013729; BC039177 

Genbank Nucleotide ID

 BAA84083.1; AAD51029.1; BAB28976.1; AAH39177.1 

Protein Name

 Ubiquitin carboxyl-terminal hydrolase isozyme L1 

Protein Synonyms/Alias

 UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1 

Gene Name

 Uchl1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
4	****MQLKPMEINPE	ubiquitination	[1]
15	INPEMLNKVLAKLGV	ubiquitination	[1]
19	MLNKVLAKLGVAGQW	acetylation	[2]
19	MLNKVLAKLGVAGQW	ubiquitination	[1]
71	KKQIEELKGQEVSPK	ubiquitination	[1]
78	KGQEVSPKVYFMKQT	ubiquitination	[1]
123	QFLSETEKLSPEDRA	acetylation	[2]
123	QFLSETEKLSPEDRA	ubiquitination	[1]
135	DRAKCFEKNEAIQAA	acetylation	[2]
135	DRAKCFEKNEAIQAA	ubiquitination	[1]
199	SLLQDAAKVCREFTE	acetylation	[2]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. 

Sequence Annotation

 REGION 5 10 Interaction with ubiquitin (By
 REGION 151 156 Interaction with ubiquitin (By
 REGION 211 216 Interaction with ubiquitin (By
 ACT_SITE 90 90 Nucleophile (Probable).
 ACT_SITE 161 161 Proton donor (By similarity).
 LIPID 220 220 S-farnesyl cysteine (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; Hydrolase; Ligase; Lipoprotein; Membrane; Oxidation; Prenylation; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 223 AA 

Protein Sequence

Gene Ontology

 GO:0030424; C:axon; IDA:MGI.
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0043025; C:neuronal cell body; IDA:MGI.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0004197; F:cysteine-type endopeptidase activity; ISS:UniProtKB.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0008242; F:omega peptidase activity; ISS:UniProtKB.
 GO:0043130; F:ubiquitin binding; ISS:UniProtKB.
 GO:0004221; F:ubiquitin thiolesterase activity; IDA:MGI.
 GO:0007628; P:adult walking behavior; IMP:MGI.
 GO:0007412; P:axon target recognition; IMP:MGI.
 GO:0019896; P:axon transport of mitochondrion; IMP:MGI.
 GO:0008283; P:cell proliferation; IMP:MGI.
 GO:0042755; P:eating behavior; IGI:MGI.
 GO:0048747; P:muscle fiber development; IGI:MGI.
 GO:0043407; P:negative regulation of MAP kinase activity; IEA:Compara.
 GO:0050905; P:neuromuscular process; IMP:MGI.
 GO:0016579; P:protein deubiquitination; ISS:UniProtKB.
 GO:0002931; P:response to ischemia; IMP:MGI.
 GO:0019233; P:sensory perception of pain; IEA:Compara.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:Compara. 

Interpro

 IPR001578; Peptidase_C12. 

Pfam

 PF01088; Peptidase_C12 

SMART

  

PROSITE

 PS00140; UCH_1 

PRINTS

 PR00707; UBCTHYDRLASE.