| Tag | Content |
|---|
| CPLM ID | CPLM-019057 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Crossover junction endonuclease EME1 |
Protein Synonyms/Alias | MMS4 homolog; hMMS4 |
Gene Name | EME1 |
Gene Synonyms/Alias | MMS4 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 241 | AALVTRMKAQRPEEC | ubiquitination | [1] | | 329 | VSMIDNGKQGSLDST | ubiquitination | [1] | | 338 | GSLDSTMKGKETLQG | ubiquitination | [1] | | 340 | LDSTMKGKETLQGFV | ubiquitination | [1] | | 353 | FVTDITAKTAGKALS | ubiquitination | [1] | | 357 | ITAKTAGKALSLVIV | ubiquitination | [1] | | 454 | DFTCAFTKAVAEAPF | ubiquitination | [1] | | 463 | VAEAPFKKLRDETTF | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'- flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. |
Sequence Annotation | MOD_RES 12 12 Phosphoserine.
MOD_RES 15 15 Phosphoserine.
MOD_RES 84 84 Phosphoserine.
MOD_RES 85 85 Phosphoserine.
MOD_RES 87 87 Phosphoserine.
MOD_RES 111 111 Phosphoserine.
MOD_RES 117 117 Phosphoserine.
MOD_RES 150 150 Phosphothreonine. |
Keyword | 3D-structure; Alternative splicing; Complete proteome; DNA damage; DNA recombination; DNA repair; Endonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 570 AA |
Protein Sequence | MALKKSSPSL DSGDSDSEEL PTFAFLKKEP SSTKRRQPER EEKIVVVDIS DCEASCPPAP 60
ELFSPPVPEI AETVTQTQPV RLLSSESEDE EEFIPLAQRL TCKFLTHKQL SPEDSSSPVK 120
SVLDHQNNEG ASCDWKKPFP KIPEVPLHDT PERSAADNKD LILDPCCQLP AYLSTCPGQS 180
SSLAVTKTNS DILPPQKKTK PSQKVQGRGS HGCRQQRQAR QKESTLRRQE RKNAALVTRM 240
KAQRPEECLK HIIVVLDPVL LQMEGGGQLL GALQTMECRC VIEAQAVPCS VTWRRRAGPS 300
EDREDWVEEP TVLVLLRAEA FVSMIDNGKQ GSLDSTMKGK ETLQGFVTDI TAKTAGKALS 360
LVIVDQEKCF SLELLFFDFL PCTSAQNPPR RGKQGANKQT KKQQQRQPEA SIGSMVSRVD 420
AEEALVDLQL HTEAQAQIVQ SWKELADFTC AFTKAVAEAP FKKLRDETTF SFCLESDWAG 480
GVKVDLAGRG LALVWRRQIQ QLNRVSLEMA SAVVNAYPSP QLLVQAYQQC FSDKERQNLL 540
ADIQVRRGEG VTSTSRRIGP ELSRRIYLQM TTLQPHLSLD SAD 583 |
Gene Ontology | GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell. GO:0003677; F:DNA binding; IEA:InterPro. GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0006310; P:DNA recombination; IEA:UniProtKB-KW. GO:0006281; P:DNA repair; IEA:UniProtKB-KW. GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |