CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042868
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Nuclear mitotic apparatus protein 1 
Protein Synonyms/Alias
  
Gene Name
 NUMA1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
175EELGQELKAWQEKFFubiquitination[1, 2, 3]
180ELKAWQEKFFQKEQAubiquitination[1]
275AQQLRAEKASYAEQLubiquitination[1]
286AEQLSMLKKAHGLLAubiquitination[3]
287EQLSMLKKAHGLLAEubiquitination[4, 5]
320ELDQAREKYVQELAAubiquitination[1, 4]
356ELEVMTAKYEGAKVKubiquitination[1, 4, 5]
404KVQQQKLKAVQAQGGubiquitination[2, 3, 4]
530QQLRDLGKFQVATDAubiquitination[1, 4, 5]
539QVATDALKSREPQAKubiquitination[1, 2, 3, 4, 5]
589SPNNPLSKLPRTQPDubiquitination[4, 5]
615ISQRLPPKVESLESLubiquitination[1, 3, 5]
670IINITMTKKLDVEEPubiquitination[1]
671INITMTKKLDVEEPDubiquitination[4, 5]
784RVCPPHLKTCYPLESubiquitination[5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 964 AA 
Protein Sequence
XRAERDSALE TLQGQLEEKA QELGHSQSAL ASAQRELAAF RTKVQDHSKA EDEWKAQVAR 60
GRQEAERKNS LISSLEEEVS ILNRQVLEKE GESKELKRLV MAESEKSQKL EERLRLLQAE 120
TASNSARAAE RSSALREEVQ SLREEAEKQR VASENLRQEL TSQAERAEEL GQELKAWQEK 180
FFQKEQALST LQLEHTSTQA LVSELLPAKH LCQQLQAEQA AAEKRHREEL EQSKQAAGGL 240
RAELLRAQRE LGELIPLRQK VAEQERTAQQ LRAEKASYAE QLSMLKKAHG LLAEENRGLG 300
ERANLGRQFL EVELDQAREK YVQELAAVRA DAETRLAEVQ REAQSTAREL EVMTAKYEGA 360
KVKVLEERQR FQEERQKLTA QVEELSKKLA DSDQASKVQQ QKLKAVQAQG GESQQEAQRL 420
QAQLNELQAQ LSQKEQAAEH YKLQMEKAKT HYDAKKQQNQ ELQEQLRSLE QLQKENKELR 480
AEAERLGHEL QQAGLKTKEA EQTCRHLTAQ VRSLEAQVAH ADQQLRDLGK FQVATDALKS 540
REPQAKPQLD LSIDSLDLSC EEGTPLSITR HKALMTIIPD LSPNNPLSKL PRTQPDGTSV 600
PGEPASPISQ RLPPKVESLE SLYFTPIPAR SQAPLESSLD SLGDVFLDSG RKTRSARRRT 660
TQIINITMTK KLDVEEPDSA NSSFYSTRSA PASQASLRAT SSTQSLARLG SPDYGNSALL 720
SLPGYRPTTR SSARRSQAGV SSGAPPGRNS FYMGTCQDEP EQLDDWNRIA ELQQRNRVCP 780
PHLKTCYPLE SRPSLSLGTI TDEEMKTGDP QETLRRASMQ PIQIAEGTGI TTRQQRKRVS 840
LEPHQGPGTP ESKKATSCFP RPMTPRDRHE GRKQSTTEAQ KKAAPASTKQ ADRRQSMAFS 900
ILNTPKKLGN SLLRRGASKK ALSKASPNTR SGTRRSPRIA TTTASAATAA AIGATPRAKG 960
KAKH 964 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA. 
Interpro
  
Pfam
  
SMART
  
PROSITE
  
PRINTS