CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037100
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Helicase ARIP4 
Protein Synonyms/Alias
  
Gene Name
 RAD54L2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
66EVQPRFFKVHILNDEubiquitination[1]
75HILNDEHKTMASRAKubiquitination[1]
144EFRREFEKALCRPGPubiquitination[1]
163CDEGHRIKNCQASTSubiquitination[1]
174ASTSQALKNIRSRRRubiquitination[1, 2]
268LKIHLPAKEENVILVubiquitination[1]
307WLGLNPLKAFCVCCKubiquitination[1]
314KAFCVCCKIWNHPDVubiquitination[1]
357RCPPQGTKGKGEDSTubiquitination[1]
359PPQGTKGKGEDSTLAubiquitination[1, 2, 3, 4, 5]
376MGEATNSKFLQGVGFubiquitination[1, 3]
469TEGQGAQKWVRNISYubiquitination[1]
550RVYRYGQKKPCYIYRubiquitination[1, 5]
551VYRYGQKKPCYIYRLubiquitination[1]
566VADYTLEKKIYDRQIubiquitination[1, 2]
567ADYTLEKKIYDRQISubiquitination[1]
575IYDRQISKQGMSDRVubiquitination[1, 5]
595PMLNFTRKEVENLLHubiquitination[1]
606NLLHFVEKEPAPQVSubiquitination[1]
617PQVSLNVKGIKESVLubiquitination[1]
620SLNVKGIKESVLQLAubiquitination[1]
630VLQLACLKYPHLITKubiquitination[5]
637KYPHLITKEPFEHESubiquitination[1]
799IHIIRGTKGTYIRTSubiquitination[1]
818FAVRATGKPKVPEDGubiquitination[3]
820VRATGKPKVPEDGRMubiquitination[1, 3, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1161 AA 
Protein Sequence
MGLGKTLQVI SFIDVLFRHT PAKTVLAIVP VNTLQNWLAE FNMWLPPPEA LPADNKPEEV 60
QPRFFKVHIL NDEHKTMASR AKVMADWVSE GGVLLMGYEM YRLLTLKKSF ATGRPKKTKK 120
RSHPVIIDLD EEDRQQEFRR EFEKALCRPG PDVVICDEGH RIKNCQASTS QALKNIRSRR 180
RVVLTGYPLQ NNLIEYWCMV DFVRPDFLGT RQEFSNMFER PILNGQCIDS TPQDVRLMRY 240
RSHVLHSLLE GFVQRRGHTV LKIHLPAKEE NVILVRLSKI QRDLYTQFMD RFRDCGSSGW 300
LGLNPLKAFC VCCKIWNHPD VLYEALQKES LANEQDLDVE ELGSAGTSAR CPPQGTKGKG 360
EDSTLASSMG EATNSKFLQG VGFNPFQERG NNIVTYEWAK DLLTNYQTGV LENSPKMVLL 420
FHLIEESVKL GDKILVFSQS LSTLALIEEF LGKREVPCPP GTEGQGAQKW VRNISYFRLD 480
GSTPAFERER LINQFNDPSN LTTWLFLLST RAGCLGVNLI GANRVVVFDA SWNPCHDAQA 540
VCRVYRYGQK KPCYIYRLVA DYTLEKKIYD RQISKQGMSD RVVDDLNPML NFTRKEVENL 600
LHFVEKEPAP QVSLNVKGIK ESVLQLACLK YPHLITKEPF EHESLLLNRK DHKLTKAEKK 660
AAKKSYEEDK RTSVPYTRPS YAQYYPASDQ SLTSIPAFSQ RNWQPTLKGD EKPVASVRPV 720
QSTPIPMMPR HVPLGGSVSS ASSTNPSMNF PINYLQRAGV LVQKVVTTTD IVIPGLNSST 780
DVQARINAGE SIHIIRGTKG TYIRTSDGRI FAVRATGKPK VPEDGRMAAS GSQGPSCEST 840
SNGRHSASSP KAPDPEGLAR PVSPDSPEII SELQQYADVA AARESRQSSP STNAALPGPP 900
AQLMDSSAVP GTALGTEPRL GGHCLNSSLL VTGQPCGDRH PVLDLRGHKR KLATPPAAQE 960
SSRRRSRKGH LPAPVQPYEH GYPVSGGFAM PPVSLNHNLT TPFTSQAGEN SLFMGSTPSY 1020
YQLSNLLADA RLVFPVTTDP LVPAGPVSSS STATSVTASN PSFMLNPSVP GILPSYSLPF 1080
SQPLLSEPRM FAPFPSPVLP SNLSRGMSIY PGYMSPHAGY PAGGLLRSQV PPFDSHEVAE 1140
VGFSSNDDED KDDDVIEVTG K 1161 
Gene Ontology
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0004386; F:helicase activity; IEA:InterPro.
 GO:0003712; F:transcription cofactor activity; IEA:Compara. 
Interpro
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000330; SNF2_N. 
Pfam
 PF00271; Helicase_C
 PF00176; SNF2_N 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS