CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008107
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Asparagine synthetase [glutamine-hydrolyzing] 1 
Protein Synonyms/Alias
 Glutamine-dependent asparagine synthetase 1 
Gene Name
 ASN1 
Gene Synonyms/Alias
 YPR145W; P9659.3 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
154GRSSASPKTVYFASEubiquitination[1]
200TPDWLDEKRIPSTPIacetylation[2]
218AIRHSLEKAVRKRLMacetylation[2]
218AIRHSLEKAVRKRLMubiquitination[1]
253IAARETAKATNDVEPubiquitination[1]
266EPSTYDSKARHLAGIubiquitination[1]
398TESVQRVKNLHLADCubiquitination[1]
451PKEGRIEKYILRKAFacetylation[2]
456IEKYILRKAFDTTGEubiquitination[1]
467TTGEPDAKPYLPEEIubiquitination[1]
530EAFWYRLKFDALFPQacetylation[2]
530EAFWYRLKFDALFPQubiquitination[1]
550TVMRWIPKADWGCAEubiquitination[1]
569RYAQIHEKHIE****acetylation[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
  
Sequence Annotation
 DOMAIN 2 186 Glutamine amidotransferase type-2.
 DOMAIN 194 546 Asparagine synthetase.
 NP_BIND 366 367 ATP (By similarity).
 REGION 49 53 Glutamine binding (By similarity).
 REGION 74 76 Glutamine binding (By similarity).
 ACT_SITE 2 2 For GATase activity (By similarity).
 BINDING 97 97 Glutamine (By similarity).
 BINDING 233 233 ATP; via carbonyl oxygen (By similarity).
 BINDING 292 292 ATP; via amide nitrogen and carbonyl
 MOD_RES 265 265 Phosphoserine.
 MOD_RES 509 509 Phosphoserine.  
Keyword
 Amino-acid biosynthesis; Asparagine biosynthesis; ATP-binding; Complete proteome; Glutamine amidotransferase; Ligase; Nucleotide-binding; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 572 AA 
Protein Sequence
MCGIFAAFRH EDVHRYKPKA LQLSKRIRHR GPDWSGNAIK NSTIFVHERL AIVGVESGAQ 60
PITSSDGEYM LCVNGEIYNH IQLREECADY EFGTLSDCEP IIPMYLKHDI DAPKYLDGMF 120
AWTLYDAKQD RIVAARDPIG ITTLYMGRSS ASPKTVYFAS ELKCLTDDCD TITAFPPGHV 180
YDSKTDKITR YFTPDWLDEK RIPSTPIDYM AIRHSLEKAV RKRLMAEVPY GVLLSGGLDS 240
SLIASIAARE TAKATNDVEP STYDSKARHL AGIDDDGKLH TAGWTSLHSF AIGLPNAPDL 300
QAARKVAKFI GSIHHEHTFT LQEGLDALDD VIYHLETYDV TTIRASTPMF LLSRKIKAQG 360
VKMVLSGEGS DEIFGGYLYF AQAPSAAEFH TESVQRVKNL HLADCLRANK STMAWGLEAR 420
VPFLDREFLQ LCMNIDPNEK MIKPKEGRIE KYILRKAFDT TGEPDAKPYL PEEILWRQKE 480
QFSDGVGYSW IDGLKDTAEA VISDEMFASP KAEWGSDIPT TKEAFWYRLK FDALFPQKTV 540
ADTVMRWIPK ADWGCAEDPS GRYAQIHEKH IE 572 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0004066; F:asparagine synthase (glutamine-hydrolyzing) activity; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006529; P:asparagine biosynthetic process; IGI:SGD.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
 GO:0070981; P:L-asparagine biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR006426; Asn_synth_AEB.
 IPR001962; Asn_synthase.
 IPR017932; GATase_2_dom.
 IPR000583; GATase_dom.
 IPR014729; Rossmann-like_a/b/a_fold. 
Pfam
 PF00733; Asn_synthase
 PF13537; GATase_7 
SMART
  
PROSITE
 PS51278; GATASE_TYPE_2 
PRINTS