UniProt Accession

 EIF3L_MOUSE; Q8QZY1; Q3THF8; Q3TJ04; Q91YE4 

Genbank Protein ID

 AJ310346; AB066095; AK032936; AK075584; AK150895; AK151175; AK167640; AK168009; AK168295; BC024463 

Genbank Nucleotide ID

 CAC84554.1; BAB85122.1; BAC28090.1; BAC35837.1; BAE29941.1; BAE30177.1; BAE39691.1; BAE39996.1; BAE40238.1; AAH24463.1 

Protein Name

 Eukaryotic translation initiation factor 3 subunit L 

Protein Synonyms/Alias

 eIF3l; 66 kDa tyrosine-rich heat shock protein; 67 kDa polymerase-associated factor; Eukaryotic translation initiation factor 3 subunit 6-interacting protein; Eukaryotic translation initiation factor 3 subunit E-interacting protein; HSP-66Y; PAF67 

Gene Name

 Eif3l 

Gene Synonyms/Alias

 Eif3eip; Eif3s6ip; Paf67 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
101	IYENSWTKLTERFFK	ubiquitination	[1]
207	YRCKTAKKSEGEMDF	ubiquitination	[1]
301	LENIELNKKSMYSRV	acetylation	[2]
403	DKMLRMQKGDPQVYE	ubiquitination	[1]
437	NVHPNYHKEPFLQQL	acetylation	[2]
437	NVHPNYHKEPFLQQL	ubiquitination	[1]
465	STIRSFLKLYTTMPV	acetylation	[2, 3]
465	STIRSFLKLYTTMPV	ubiquitination	[1]
549	FFIRQIHKFEELNRT	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. 

Sequence Annotation

 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 465 465 N6-acetyllysine (By similarity).
 MOD_RES 549 549 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Initiation factor; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 564 AA 

Protein Sequence

Gene Ontology

 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IDA:UniProtKB.
 GO:0001650; C:fibrillar center; IDA:MGI.
 GO:0005654; C:nucleoplasm; IDA:MGI.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP.
 GO:0006413; P:translational initiation; IDA:UniProtKB. 

Interpro

 IPR019382; eIF3l. 

Pfam

 PF10255; Paf67 

SMART

  

PROSITE

  

PRINTS