UniProt Accession

 IF5A1_MOUSE; P63242; P10159; Q16182; Q5NCX0; Q78NR3; Q9D0G2 

Genbank Protein ID

 AY129323; AY129324; AY129325; AY129326; AY129327; AY129328; AY129329; AK011306; AK011470; AK149705; AL596185; AL596185; BC003889; BC008093; BC024899 

Genbank Nucleotide ID

 AAN17521.1; AAN17527.1; AAN17528.1; AAN17532.1; AAN17534.1; AAN17535.1; AAN17539.1; BAB27532.1; BAB27641.1; BAE29039.1; CAI35153.1; CAI35154.1; AAH03889.1; AAH08093.1; AAH24899.1 

Protein Name

 Eukaryotic translation initiation factor 5A-1 

Protein Synonyms/Alias

 eIF-5A-1; eIF-5A1; Eukaryotic initiation factor 5A isoform 1; eIF-5A; eIF-4D 

Gene Name

 Eif5a 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
27	MQCSALRKNGFVVLK	ubiquitination	[1]
34	KNGFVVLKGRPCKIV	acetylation	[2]
39	VLKGRPCKIVEMSTS	acetylation	[2]
39	VLKGRPCKIVEMSTS	ubiquitination	[1]
47	IVEMSTSKTGKHGHA	acetylation	[2, 3, 4, 5]
67	GIDIFTGKKYEDICP	ubiquitination	[1]
68	IDIFTGKKYEDICPS	acetylation	[2, 5]
68	IDIFTGKKYEDICPS	succinylation	[5]
68	IDIFTGKKYEDICPS	ubiquitination	[1]
85	NMDVPNIKRNDFQLI	acetylation	[2]
85	NMDVPNIKRNDFQLI	ubiquitination	[1]
121	LPEGDLGKEIEQKYD	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF- alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity). 

Sequence Annotation

 REGION 2 19 Nuclear localization regulation.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 47 47 N6-acetyllysine (By similarity).
 MOD_RES 50 50 Hypusine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Elongation factor; Endoplasmic reticulum; Hypusine; Membrane; mRNA transport; Nuclear pore complex; Nucleus; Protein biosynthesis; Protein transport; Reference proteome; RNA-binding; Translocation; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 154 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0030425; C:dendrite; IEA:Compara.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005643; C:nuclear pore; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0043022; F:ribosome binding; IEA:InterPro.
 GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
 GO:0007568; P:aging; IEA:Compara.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0008612; P:peptidyl-lysine modification to hypusine; IEA:InterPro.
 GO:0043065; P:positive regulation of apoptotic process; IDA:MGI.
 GO:0051149; P:positive regulation of muscle cell differentiation; IEA:Compara.
 GO:0045901; P:positive regulation of translational elongation; IEA:InterPro.
 GO:0045905; P:positive regulation of translational termination; IEA:InterPro.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0006452; P:translational frameshifting; IEA:InterPro. 

Interpro

 IPR005824; KOW.
 IPR012340; NA-bd_OB-fold.
 IPR014722; Rib_L2_dom2.
 IPR019769; Trans_elong_IF5A_hypusine_site.
 IPR001884; Transl_elong_IF5A.
 IPR020189; Transl_elong_IF5A_C.
 IPR008991; Translation_prot_SH3-like. 

Pfam

 PF01287; eIF-5a
 PF00467; KOW 

SMART

  

PROSITE

 PS00302; IF5A_HYPUSINE 

PRINTS