UniProt Accession

 HRR25_YEAST; P29295; D6W3G5 

Genbank Protein ID

 M68605; Z73560; BK006949 

Genbank Nucleotide ID

 AAA34687.1; CAA97918.1; DAA11231.1 

Protein Name

 Casein kinase I homolog HRR25 

Protein Synonyms/Alias

  

Gene Name

 HRR25 

Gene Synonyms/Alias

 YPL204W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
14	RKFRIGRKIGSGSFG	ubiquitination	[1]
259	MAYCKNLKFDEKPDY	acetylation	[2]
310	QRDLLIEKGDLNANS	ubiquitination	[1]
329	ASNSTDNKSETFNKI	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Associated with repair of damaged DNA and meiosis. Phosphorylates serine and threonine. Can use casein as a substrate. 

Sequence Annotation

 DOMAIN 9 278 Protein kinase.
 NP_BIND 15 23 ATP (By similarity).
 ACT_SITE 128 128 Proton acceptor (By similarity).
 BINDING 38 38 ATP (By similarity).
 MOD_RES 143 143 Phosphoserine.  

Keyword

 ATP-binding; Complete proteome; Cytoplasm; DNA damage; DNA repair; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 494 AA 

Protein Sequence

Gene Ontology

 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0005934; C:cellular bud tip; IDA:SGD.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0033551; C:monopolin complex; IDA:SGD.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005816; C:spindle pole body; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:SGD.
 GO:0051455; P:attachment of spindle microtubules to kinetochore involved in homologous chromosome segregation; IMP:SGD.
 GO:0006281; P:DNA repair; IMP:SGD.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IMP:SGD.
 GO:0007067; P:mitosis; IMP:SGD.
 GO:0042274; P:ribosomal small subunit biogenesis; IMP:SGD.
 GO:0002098; P:tRNA wobble uridine modification; IMP:SGD. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

  

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS