UniProt Accession

 PP12_YEAST; P32598; D3DM39 

Genbank Protein ID

 M77175; M27070; U18916; BK006939 

Genbank Nucleotide ID

 AAB59322.1; AAA34570.1; AAC03231.1; DAA07793.1 

Protein Name

 Serine/threonine-protein phosphatase PP1-2 

Protein Synonyms/Alias

  

Gene Name

 GLC7 

Gene Synonyms/Alias

 CID1; DIS2; YER133W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
22	LLEVRGSKPGQQVDL	ubiquitination	[1, 2]
40	EIRYLCSKARSIFIK	ubiquitination	[2]
47	KARSIFIKQPILLEL	ubiquitination	[1]
112	LLLAYKIKYPENFFI	ubiquitination	[2]
146	CKRRYNIKLWKTFTD	acetylation	[3]
146	CKRRYNIKLWKTFTD	ubiquitination	[2]
149	RYNIKLWKTFTDCFN	ubiquitination	[2]
237	VVNRFLQKQDMELIC	ubiquitination	[2]
259	DGYEFFSKRQLVTLF	acetylation	[3]
259	DGYEFFSKRQLVTLF	ubiquitination	[2]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Involved in control of glycogen metabolism, meiosis, translation, chromosome segregation, cell polarity and G2/M cell cycle progression. PP1 may act in opposition to the IPL1 protein kinase in regulating chromosome segregation by dephosphorylating H3S10ph. The BUD14-GLC7 complex is necessary to regulate microtubule dynamics at the cortex and may function as a specific activator of the dynein complex. 

Sequence Annotation

 ACT_SITE 124 124 Proton donor (By similarity).
 METAL 63 63 Iron (By similarity).
 METAL 65 65 Iron (By similarity).
 METAL 91 91 Iron (By similarity).
 METAL 91 91 Manganese (By similarity).
 METAL 123 123 Manganese (By similarity).
 METAL 172 172 Manganese (By similarity).
 METAL 247 247 Manganese (By similarity).  

Keyword

 Carbohydrate metabolism; Cell cycle; Cell division; Complete proteome; Cytoplasm; Glycogen metabolism; Hydrolase; Iron; Manganese; Metal-binding; Mitosis; mRNA processing; Nucleus; Protein phosphatase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 312 AA 

Protein Sequence

Gene Ontology

 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0000778; C:condensed nuclear chromosome kinetochore; IDA:SGD.
 GO:0001400; C:mating projection base; IDA:SGD.
 GO:0005847; C:mRNA cleavage and polyadenylation specificity factor complex; IDA:SGD.
 GO:0005730; C:nucleolus; IDA:SGD.
 GO:0000164; C:protein phosphatase type 1 complex; IDA:SGD.
 GO:0005816; C:spindle pole body; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004722; F:protein serine/threonine phosphatase activity; IDA:SGD.
 GO:0030437; P:ascospore formation; IMP:SGD.
 GO:0007114; P:cell budding; IMP:SGD.
 GO:0006873; P:cellular ion homeostasis; IMP:SGD.
 GO:0007059; P:chromosome segregation; IMP:SGD.
 GO:0000077; P:DNA damage checkpoint; IMP:SGD.
 GO:0000076; P:DNA replication checkpoint; IMP:SGD.
 GO:0005977; P:glycogen metabolic process; IMP:SGD.
 GO:0016576; P:histone dephosphorylation; IDA:SGD.
 GO:0007126; P:meiosis; IPI:SGD.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0007094; P:mitotic spindle assembly checkpoint; IMP:SGD.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0034501; P:protein localization to kinetochore; IMP:SGD.
 GO:0006109; P:regulation of carbohydrate metabolic process; IGI:SGD.
 GO:0000903; P:regulation of cell shape during vegetative growth phase; IGI:SGD.
 GO:0031297; P:replication fork processing; IMP:SGD.
 GO:0009408; P:response to heat; IPI:SGD.
 GO:0006364; P:rRNA processing; IMP:SGD.
 GO:0030847; P:termination of RNA polymerase II transcription, exosome-dependent; IPI:SGD.
 GO:0030846; P:termination of RNA polymerase II transcription, poly(A)-coupled; IPI:SGD. 

Interpro

 IPR004843; Metallo_PEstase_dom.
 IPR006186; Ser/Thr-sp_prot-phosphatase. 

Pfam

 PF00149; Metallophos 

SMART

 SM00156; PP2Ac 

PROSITE

 PS00125; SER_THR_PHOSPHATASE 

PRINTS

 PR00114; STPHPHTASE.