UniProt Accession

 MB12B_HUMAN; Q9H7P6; Q8N6S7 

Genbank Protein ID

 AK024432; BC028675 

Genbank Nucleotide ID

 BAB15722.1; AAH28675.1 

Protein Name

 Multivesicular body subunit 12B 

Protein Synonyms/Alias

 ESCRT-I complex subunit MVB12B; Protein FAM125B 

Gene Name

 MVB12B 

Gene Synonyms/Alias

 C9orf28; FAM125B 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
102	CFTRSFSKENSHLGN	ubiquitination	[1]
115	GNVLVDMKLIDIKDT	ubiquitination	[2, 3]
290	KSLAEIEKEYEYSFR	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. 

Sequence Annotation

 DOMAIN 47 193 MABP.
 DOMAIN 254 303 UMA.
 MOD_RES 46 46 Phosphoserine.
 MOD_RES 101 101 Phosphoserine.
 MOD_RES 122 122 Phosphothreonine.
 MOD_RES 204 204 Phosphothreonine.
 MOD_RES 205 205 Phosphothreonine.
 MOD_RES 309 309 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Endosome; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 319 AA 

Protein Sequence

Gene Ontology

 GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 

Interpro

 IPR018798; FAM125.
 IPR023341; MABP.
 IPR023340; UMA. 

Pfam

 PF10240; DUF2464 

SMART

  

PROSITE

 PS51498; MABP
 PS51497; UMA 

PRINTS