CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009067
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Beta-secretase 1 
Protein Synonyms/Alias
 Aspartyl protease 2; ASP2; Asp 2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2 
Gene Name
 BACE1 
Gene Synonyms/Alias
 BACE; KIAA1149 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
275EINGQDLKMDCKEYNsumoylation[1]
Reference
 [1] Positive and negative regulation of APP amyloidogenesis by sumoylation.
 Li Y, Wang H, Wang S, Quon D, Liu YW, Cordell B.
 Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):259-64. [PMID: 12506199] 
Functional Description
 Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. 
Sequence Annotation
 REGION 479 501 Interaction with RTN3.
 ACT_SITE 93 93 By similarity.
 ACT_SITE 289 289 By similarity.
 CARBOHYD 153 153 N-linked (GlcNAc...) (Potential).
 CARBOHYD 172 172 N-linked (GlcNAc...) (Potential).
 CARBOHYD 223 223 N-linked (GlcNAc...) (Potential).
 CARBOHYD 354 354 N-linked (GlcNAc...) (Potential).
 DISULFID 216 420
 DISULFID 278 443
 DISULFID 330 380  
Keyword
 3D-structure; Alternative splicing; Aspartyl protease; Complete proteome; Cytoplasmic vesicle; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Endosome; Glycoprotein; Golgi apparatus; Hydrolase; Membrane; Polymorphism; Protease; Reference proteome; Signal; Transmembrane; Transmembrane helix; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 501 AA 
Protein Sequence
MAQALPWLLL WMGAGVLPAH GTQHGIRLPL RSGLGGAPLG LRLPRETDEE PEEPGRRGSF 60
VEMVDNLRGK SGQGYYVEMT VGSPPQTLNI LVDTGSSNFA VGAAPHPFLH RYYQRQLSST 120
YRDLRKGVYV PYTQGKWEGE LGTDLVSIPH GPNVTVRANI AAITESDKFF INGSNWEGIL 180
GLAYAEIARP DDSLEPFFDS LVKQTHVPNL FSLQLCGAGF PLNQSEVLAS VGGSMIIGGI 240
DHSLYTGSLW YTPIRREWYY EVIIVRVEIN GQDLKMDCKE YNYDKSIVDS GTTNLRLPKK 300
VFEAAVKSIK AASSTEKFPD GFWLGEQLVC WQAGTTPWNI FPVISLYLMG EVTNQSFRIT 360
ILPQQYLRPV EDVATSQDDC YKFAISQSST GTVMGAVIME GFYVVFDRAR KRIGFAVSAC 420
HVHDEFRTAA VEGPFVTLDM EDCGYNIPQT DESTLMTIAY VMAAICALFM LPLCLMVCQW 480
CCLRCLRQQH DDFADDISLL K 501 
Gene Ontology
 GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
 GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005768; C:endosome; IDA:UniProtKB.
 GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
 GO:0005887; C:integral to plasma membrane; IDA:UniProtKB.
 GO:0005802; C:trans-Golgi network; IDA:UniProtKB.
 GO:0004190; F:aspartic-type endopeptidase activity; IDA:UniProtKB.
 GO:0008798; F:beta-aspartyl-peptidase activity; TAS:ProtInc.
 GO:0050435; P:beta-amyloid metabolic process; IDA:UniProtKB.
 GO:0006509; P:membrane protein ectodomain proteolysis; TAS:UniProtKB. 
Interpro
 IPR009119; Pept_A1_BACE.
 IPR009120; Pept_A1_BACE1.
 IPR001461; Peptidase_A1.
 IPR021109; Peptidase_aspartic.
 IPR001969; Peptidase_aspartic_AS. 
Pfam
 PF00026; Asp 
SMART
  
PROSITE
 PS00141; ASP_PROTEASE 
PRINTS
 PR01816; BACE1.
 PR01815; BACEFAMILY.
 PR00792; PEPSIN.