UniProt Accession

 PAPOA_MOUSE; Q61183; Q61208; Q61209; Q8K4X2 

Genbank Protein ID

 U52197; U58134; U58135; AB086650 

Genbank Nucleotide ID

 AAC52586.1; AAC52608.1; AAC52609.1; BAC00996.1 

Protein Name

 Poly(A) polymerase alpha 

Protein Synonyms/Alias

 PAP-alpha; Polynucleotide adenylyltransferase 

Gene Name

 Papola 

Gene Synonyms/Alias

 Pap; Plap 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
444	WVIGLVFKKTENSEN	sumoylation	[1]
445	VIGLVFKKTENSENL	sumoylation	[1]
506	SHVLQKRKKHSTEGV	sumoylation	[1]
507	HVLQKRKKHSTEGVK	sumoylation	[1]
730	ANPIPVIKNSIKLRL	sumoylation	[1]
734	PVIKNSIKLRLNR**	sumoylation	[1]

Reference

 [1] Sumoylation regulates multiple aspects of mammalian poly(A) polymerase function.
 Vethantham V, Rao N, Manley JL.
 Genes Dev. 2008 Feb 15;22(4):499-511. [PMID: 18281463] 

Functional Description

 Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus (By similarity). 

Sequence Annotation

 NP_BIND 100 102 ATP (By similarity).
 NP_BIND 113 115 ATP (By similarity).
 NP_BIND 246 247 ATP (By similarity).
 REGION 508 643 Ser/Thr-rich.
 REGION 671 739 Required for interaction with NUDT21.
 MOTIF 490 507 Nuclear localization signal 1 (By
 MOTIF 644 659 Nuclear localization signal 2 (By
 METAL 113 113 Magnesium 1; catalytic (By similarity).
 METAL 113 113 Magnesium 2; catalytic (By similarity).
 METAL 115 115 Magnesium 1; catalytic (By similarity).
 METAL 115 115 Magnesium 2; catalytic (By similarity).
 METAL 167 167 Magnesium 2; catalytic (By similarity).
 BINDING 109 109 ATP (By similarity).
 BINDING 167 167 ATP (By similarity).
 BINDING 228 228 ATP (By similarity).
 BINDING 237 237 ATP (By similarity).
 MOD_RES 24 24 Phosphoserine (By similarity).
 MOD_RES 537 537 Phosphoserine; by MAPK.
 MOD_RES 635 635 N6-acetyllysine (By similarity).
 MOD_RES 644 644 N6-acetyllysine (By similarity).
 MOD_RES 730 730 N6-acetyllysine; alternate (By
 MOD_RES 734 734 N6-acetyllysine; alternate (By
 CROSSLNK 444 444 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 445 445 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 506 506 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 507 507 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 730 730 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 734 734 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Alternative splicing; ATP-binding; Complete proteome; Isopeptide bond; Magnesium; Manganese; Metal-binding; mRNA processing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Transferase; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 739 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IBA:RefGenome.
 GO:0005634; C:nucleus; IBA:RefGenome.
 GO:0005524; F:ATP binding; ISS:UniProtKB.
 GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
 GO:0030145; F:manganese ion binding; ISS:UniProtKB.
 GO:0004652; F:polynucleotide adenylyltransferase activity; ISS:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006378; P:mRNA polyadenylation; IBA:RefGenome.
 GO:0000398; P:mRNA splicing, via spliceosome; IBA:RefGenome.
 GO:0006369; P:termination of RNA polymerase II transcription; IBA:RefGenome. 

Interpro

 IPR002934; Nucleotidyltransferase.
 IPR011068; NuclTrfase_I_C.
 IPR007012; PolA_pol_cen_dom.
 IPR007010; PolA_pol_RNA-bd_dom.
 IPR014492; PolyA_polymerase. 

Pfam

 PF01909; NTP_transf_2
 PF04928; PAP_central
 PF04926; PAP_RNA-bind 

SMART

  

PROSITE

  

PRINTS