UniProt Accession

 B4DF52_HUMAN; B4DF52 

Genbank Protein ID

 AL137145; AL158043; AK293935 

Genbank Nucleotide ID

 BAG57313.1 

Protein Name

 Protein kinase C theta type 

Protein Synonyms/Alias

 cDNA FLJ60459, highly similar to Protein kinase C theta type (EC 2.7.11.13) 

Gene Name

 PRKCQ 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
83	CIDKVIAKCTGSAIN	ubiquitination	[1, 2]
98	SRETMFHKERFKIDM	ubiquitination	[1]
102	MFHKERFKIDMPHRF	ubiquitination	[1, 2]
110	IDMPHRFKVYNYKSP	ubiquitination	[1, 2]
115	RFKVYNYKSPTFCEH	ubiquitination	[1]
151	VHHRCQTKVANLCGI	ubiquitination	[1]
161	NLCGINQKLMAEALA	ubiquitination	[1]
200	IGLPCSIKNEARPPC	ubiquitination	[1, 2]
251	PSLQIKLKIEDFILH	ubiquitination	[1]
259	IEDFILHKMLGKGSF	ubiquitination	[1]
263	ILHKMLGKGSFGKVF	ubiquitination	[1]
275	KVFLAEFKKTNQFFA	ubiquitination	[1]
276	VFLAEFKKTNQFFAI	ubiquitination	[2]
304	VECTMVEKRVLSLAW	ubiquitination	[1]
381	GIVYRDLKLDNILLD	ubiquitination	[1]
389	LDNILLDKDGHIKIA	ubiquitination	[2]
402	IADFGMCKENMLGDA	ubiquitination	[1, 2, 3]
410	ENMLGDAKTNTFCGT	ubiquitination	[1]
479	FYPRWLEKEAKDLLV	ubiquitination	[1]
482	RWLEKEAKDLLVKLF	acetylation	[2]
495	LFVREPEKRLGVRGD	ubiquitination	[1]
547	DKEFLNEKPRLSFAD	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

  

Sequence Annotation

  

Keyword

 ATP-binding; Complete proteome; Kinase; Metal-binding; Nucleotide-binding; Reference proteome; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 581 AA 

Protein Sequence

Gene Ontology

 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro. 

Interpro

 IPR000961; AGC-kinase_C.
 IPR020454; DAG/PE-bd.
 IPR011009; Kinase-like_dom.
 IPR017892; Pkinase_C.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00130; C1_1
 PF00069; Pkinase
 PF00433; Pkinase_C 

SMART

 SM00109; C1
 SM00133; S_TK_X
 SM00220; S_TKc 

PROSITE

 PS51285; AGC_KINASE_CTER
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 

PRINTS

 PR00008; DAGPEDOMAIN.