CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007000
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 26S proteasome regulatory subunit RPN1 
Protein Synonyms/Alias
 HMG-CoA reductase degradation protein 2; Proteasome non-ATPase subunit 1 
Gene Name
 RPN1 
Gene Synonyms/Alias
 HRD2; NAS1; RPD1; YHR027C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
22QSQISPEKQTPNKKDubiquitination[1]
197TSGFEFSKEDTLRLCubiquitination[1]
344EHFLYLAKELNLTGPubiquitination[1]
352ELNLTGPKVPEDIYKubiquitination[1]
409DNDNWVYKTKGDGMTacetylation[2]
409DNDNWVYKTKGDGMTubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. 
Sequence Annotation
 REPEAT 417 449 PC 1.
 REPEAT 450 487 PC 2.
 REPEAT 488 522 PC 3.
 REPEAT 523 563 PC 4.
 REPEAT 566 598 PC 5.
 REPEAT 778 809 PC 6.
 REPEAT 810 844 PC 7.
 MOD_RES 16 16 Phosphoserine.
 MOD_RES 19 19 Phosphoserine.
 MOD_RES 24 24 Phosphothreonine.
 MOD_RES 178 178 Phosphoserine.
 MOD_RES 187 187 Phosphoserine.
 MOD_RES 695 695 Phosphoserine.  
Keyword
 Complete proteome; Direct protein sequencing; Phosphoprotein; Proteasome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 993 AA 
Protein Sequence
MVDESDKKQQ TIDEQSQISP EKQTPNKKDK KKEEEEQLSE EDAKLKTDLE LLVERLKEDD 60
SSLYEASLNA LKESIKNSTS SMTAVPKPLK FLRPTYPDLC SIYDKWTDPN LKSSLADVLS 120
ILAMTYSENG KHDSLRYRLL SDVSDFEGWG HEYIRHLALE IGEVYNDQVE KDAEDETSSD 180
GSKSDGSAAT SGFEFSKEDT LRLCLDIVPY FLKHNGEEDA VDLLLEIESI DKLPQFVDEN 240
TFQRVCQYMV ACVPLLPPPE DVAFLKTAYS IYLSQNELTD AIALAVRLGE EDMIRSVFDA 300
TSDPVMHKQL AYILAAQKTS FEYEGVQDII GNGKLSEHFL YLAKELNLTG PKVPEDIYKS 360
HLDNSKSVFS SAGLDSAQQN LASSFVNGFL NLGYCNDKLI VDNDNWVYKT KGDGMTSAVA 420
SIGSIYQWNL DGLQQLDKYL YVDEPEVKAG ALLGIGISAS GVHDGEVEPA LLLLQDYVTN 480
PDTKISSAAI LGLGIAFAGS KNDEVLGLLL PIAASTDLPI ETAAMASLAL AHVFVGTCNG 540
DITTSIMDNF LERTAIELKT DWVRFLALAL GILYMGQGEQ VDDVLETISA IEHPMTSAIE 600
VLVGSCAYTG TGDVLLIQDL LHRLTPKNVK GEEDADEEET AEGQTNSISD FLGEQVNEPT 660
KNEEAEIEVD EMEVDAEGEE VEVKAEITEK KNGESLEGEE IKSEEKKGKS SDKDATTDGK 720
NDDEEEEKEA GIVDELAYAV LGIALIALGE DIGKEMSLRH FGHLMHYGNE HIRRMVPLAM 780
GIVSVSDPQM KVFDTLTRFS HDADLEVSMN SIFAMGLCGA GTNNARLAQL LRQLASYYSR 840
EQDALFITRL AQGLLHLGKG TMTMDVFNDA HVLNKVTLAS ILTTAVGLVS PSFMLKHHQL 900
FYMLNAGIRP KFILALNDEG EPIKVNVRVG QAVETVGQAG RPKKITGWIT QSTPVLLNHG 960
ERAELETDEY ISYTSHIEGV VILKKNPDYR EEE 993 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0008540; C:proteasome regulatory particle, base subcomplex; IDA:SGD.
 GO:0034515; C:proteasome storage granule; IDA:SGD.
 GO:0030234; F:enzyme regulator activity; IEA:InterPro.
 GO:0050790; P:regulation of catalytic activity; IEA:GOC.
 GO:0042176; P:regulation of protein catabolic process; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; TAS:SGD. 
Interpro
 IPR016643; 26S_Psome_Rpn1.
 IPR002015; APC_proteasome.
 IPR016024; ARM-type_fold. 
Pfam
 PF01851; PC_rep 
SMART
  
PROSITE
  
PRINTS