UniProt Accession

 NCS1_HUMAN; P62166; E9PAY3; P36610; Q9UK26 

Genbank Protein ID

 X84048; AF134479; AF186409; AL360004; BC004856; BQ880305 

Genbank Nucleotide ID

 CAA58867.1; AAP97256.1; AAF01804.1; CAI16951.1; AAH04856.1 

Protein Name

 Neuronal calcium sensor 1 

Protein Synonyms/Alias

 NCS-1; Frequenin homolog; Frequenin-like protein; Frequenin-like ubiquitous protein 

Gene Name

 NCS1 

Gene Synonyms/Alias

 FLUP; FREQ 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
25	RKTYFTEKEVQQWYK	ubiquitination	[1, 2]
53	AGFQKIYKQFFPFGD	ubiquitination	[1]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N- type voltage-gated calcium channel (By similarity). 

Sequence Annotation

 DOMAIN 24 59 EF-hand 1.
 DOMAIN 60 95 EF-hand 2.
 DOMAIN 96 131 EF-hand 3.
 DOMAIN 144 179 EF-hand 4.
 REGION 174 190 Interaction with IL1RAPL1.
 LIPID 2 2 N-myristoyl glycine (By similarity).  

Keyword

 3D-structure; Alternative splicing; Calcium; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Golgi apparatus; Lipoprotein; Membrane; Metal-binding; Myristate; Postsynaptic cell membrane; Reference proteome; Repeat; Synapse. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 190 AA 

Protein Sequence

Gene Ontology

 GO:0030424; C:axon; IEA:Compara.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0031410; C:cytoplasmic vesicle; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
 GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0014069; C:postsynaptic density; IEA:UniProtKB-SubCell.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-KW.
 GO:0005509; F:calcium ion binding; TAS:UniProtKB.
 GO:0005245; F:voltage-gated calcium channel activity; ISS:UniProtKB.
 GO:0048015; P:phosphatidylinositol-mediated signaling; IEA:Compara.
 GO:0045921; P:positive regulation of exocytosis; IEA:Compara.
 GO:0010975; P:regulation of neuron projection development; ISS:UniProtKB. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR001125; Recoverin. 

Pfam

 PF00036; efhand 

SMART

 SM00054; EFh 

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 

PRINTS

 PR00450; RECOVERIN.