UniProt Accession

 GNAI1_RAT; P10824; Q45QN2 

Genbank Protein ID

 M17527; DQ120469; DQ120470 

Genbank Nucleotide ID

 AAA40825.1; AAZ23808.1; AAZ23809.1 

Protein Name

 Guanine nucleotide-binding protein G(i) subunit alpha-1 

Protein Synonyms/Alias

 Adenylate cyclase-inhibiting G alpha protein 

Gene Name

 Gnai1 

Gene Synonyms/Alias

 Gnai-1 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
29	NLREDGEKAAREVKL	ubiquitination	[1]
35	EKAAREVKLLLLGAG	ubiquitination	[1]
92	IRAMGRLKIDFGDAA	ubiquitination	[1]
271	IILFLNKKDLFEEKI	ubiquitination	[1]
277	KKDLFEEKIKKSPLT	ubiquitination	[1]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division. 

Sequence Annotation

 NP_BIND 40 47 GTP (By similarity).
 NP_BIND 43 48 GTP.
 NP_BIND 175 181 GTP (By similarity).
 NP_BIND 175 178 GTP.
 NP_BIND 200 204 GTP (By similarity).
 NP_BIND 269 272 GTP.
 METAL 47 47 Magnesium (By similarity).
 METAL 181 181 Magnesium (By similarity).
 BINDING 326 326 GTP; via amide nitrogen (By similarity).
 LIPID 2 2 N-myristoyl glycine (By similarity).
 LIPID 3 3 S-palmitoyl cysteine (By similarity).  

Keyword

 3D-structure; Cell cycle; Cell division; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; GTP-binding; Lipoprotein; Magnesium; Membrane; Metal-binding; Myristate; Nucleotide-binding; Nucleus; Palmitate; Reference proteome; Transducer. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 354 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005834; C:heterotrimeric G-protein complex; IBA:RefGenome.
 GO:0045121; C:membrane raft; IDA:RGD.
 GO:0030496; C:midbody; ISS:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:RefGenome.
 GO:0019003; F:GDP binding; IDA:RGD.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0032794; F:GTPase activating protein binding; IDA:RGD.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004871; F:signal transducer activity; IBA:RefGenome.
 GO:0007193; P:adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway; IBA:RefGenome.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; ISS:UniProtKB.
 GO:0050805; P:negative regulation of synaptic transmission; IMP:RGD. 

Interpro

 IPR001408; Gprotein_alpha_I.
 IPR001019; Gprotein_alpha_su.
 IPR011025; GproteinA_insert.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00503; G-alpha 

SMART

 SM00275; G_alpha 

PROSITE

  

PRINTS

 PR00318; GPROTEINA.
 PR00441; GPROTEINAI.