UniProt Accession

 BACD3_HUMAN; Q9H3F6; Q53HN2; Q59FV1; Q6PL47; Q96SU0 

Genbank Protein ID

 AY205299; AF113208; AY597809; AK027543; AB209358; AK222548; AC007570; BC040062 

Genbank Nucleotide ID

 AAO47716.1; AAG39279.1; AAT09002.1; BAB55188.1; BAD92595.1; BAD96268.1; AAH40062.1 

Protein Name

 BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3 

Protein Synonyms/Alias

 hBACURD3; BTB/POZ domain-containing protein KCTD10; Potassium channel tetramerization domain-containing protein 10 

Gene Name

 KCTD10 

Gene Synonyms/Alias

 ULR061; MSTP028 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
25	ATRTTSFKGTSPSSK	ubiquitination	[1, 2, 3, 4, 5, 6]
32	KGTSPSSKYVKLNVG	ubiquitination	[1, 2, 3, 5, 6]
52	TTMQTLTKQDTMLKA	ubiquitination	[2, 5, 6]
58	TKQDTMLKAMFSGRM	ubiquitination	[1, 5]
147	VPVITSSKEEQKLIA	ubiquitination	[2, 5]
151	TSSKEEQKLIATSNK	ubiquitination	[5]
158	KLIATSNKPAVKLLY	ubiquitination	[2, 5]
162	TSNKPAVKLLYNRSN	ubiquitination	[1, 5]
171	LYNRSNNKYSYTSNS	ubiquitination	[1, 2, 5, 6]
184	NSDDNMLKNIELFDK	ubiquitination	[2, 6]
191	KNIELFDKLSLRFNG	ubiquitination	[6]
303	RVRRIHIKRPDDRAH	ubiquitination	[5]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex. The BCR(BACURD3) E3 ubiquitin ligase complex mediates the ubiquitination of target proteins, leading to their degradation by the proteasome (By similarity). 

Sequence Annotation

 DOMAIN 32 100 BTB.
 MOTIF 239 245 PCNA-binding (By similarity).
 MOD_RES 23 23 Phosphoserine.  

Keyword

 Alternative splicing; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 313 AA 

Protein Sequence

Gene Ontology

 GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
 GO:0051260; P:protein homooligomerization; IEA:InterPro.
 GO:0016567; P:protein ubiquitination; ISS:UniProtKB. 

Interpro

 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR003131; T1-type_BTB. 

Pfam

 PF02214; K_tetra 

SMART

 SM00225; BTB 

PROSITE

 PS50097; BTB 

PRINTS